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Bimodal expression of PHO84 is modulated by early termination of antisense transcription.
- Source :
-
Nature structural & molecular biology [Nat Struct Mol Biol] 2013 Jul; Vol. 20 (7), pp. 851-8. Date of Electronic Publication: 2013 Jun 16. - Publication Year :
- 2013
-
Abstract
- Many Saccharomyces cerevisiae genes encode antisense transcripts, some of which are unstable and degraded by the exosome component Rrp6. Loss of Rrp6 results in the accumulation of long PHO84 antisense (AS) RNAs and repression of sense transcription through PHO84 promoter deacetylation. We used single-molecule resolution fluorescent in situ hybridization (smFISH) to investigate antisense-mediated transcription regulation. We show that PHO84 AS RNA acts as a bimodal switch, in which continuous, low-frequency antisense transcription represses sense expression within individual cells. Surprisingly, antisense RNAs do not accumulate at the PHO84 gene but are exported to the cytoplasm. Furthermore, rather than stabilizing PHO84 AS RNA, the loss of Rrp6 favors its elongation by reducing early transcription termination by Nrd1-Nab3-Sen1. These observations suggest that PHO84 silencing results from antisense transcription through the promoter rather than the static accumulation of antisense RNAs at the repressed gene.
- Subjects :
- DNA Helicases physiology
Exosome Multienzyme Ribonuclease Complex physiology
Histone Deacetylases physiology
Histone-Lysine N-Methyltransferase physiology
In Situ Hybridization, Fluorescence
Metalloendopeptidases physiology
Models, Genetic
Multiprotein Complexes
Nuclear Proteins physiology
Polyadenylation
Polynucleotide Adenylyltransferase physiology
Promoter Regions, Genetic genetics
Proton-Phosphate Symporters biosynthesis
RNA Helicases physiology
RNA, Antisense metabolism
RNA, Fungal metabolism
RNA, Messenger biosynthesis
RNA, Messenger metabolism
RNA-Binding Proteins physiology
Saccharomyces cerevisiae Proteins biosynthesis
Saccharomyces cerevisiae Proteins physiology
Gene Expression Regulation, Fungal
Proton-Phosphate Symporters genetics
RNA, Antisense genetics
RNA, Fungal genetics
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae Proteins genetics
Transcription, Genetic
Subjects
Details
- Language :
- English
- ISSN :
- 1545-9985
- Volume :
- 20
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Nature structural & molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 23770821
- Full Text :
- https://doi.org/10.1038/nsmb.2598