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Antibody responses to Sarcoptes scabiei apolipoprotein in a porcine model: relevance to immunodiagnosis of recent infection.
- Source :
-
PloS one [PLoS One] 2013 Jun 06; Vol. 8 (6), pp. e65354. Date of Electronic Publication: 2013 Jun 06 (Print Publication: 2013). - Publication Year :
- 2013
-
Abstract
- No commercial immunodiagnostic tests for human scabies are currently available, and existing animal tests are not sufficiently sensitive. The recombinant Sarcoptes scabiei apolipoprotein antigen Sar s 14.3 is a promising immunodiagnostic, eliciting high levels of IgE and IgG in infected people. Limited data are available regarding the temporal development of antibodies to Sar s 14.3, an issue of relevance in terms of immunodiagnosis. We utilised a porcine model to prospectively compare specific antibody responses to a primary infestation by ELISA, to Sar s 14.3 and to S. scabiei whole mite antigen extract (WMA). Differences in the antibody profile between antigens were apparent, with Sar s 14.3 responses detected earlier, and declining significantly after peak infestation compared to WMA. Both antigens resulted in >90% diagnostic sensitivity from weeks 8-16 post infestation. These data provide important information on the temporal development of humoral immune responses in scabies and further supports the development of recombinant antigen based immunodiagnostic tests for recent scabies infestations.
- Subjects :
- Animals
Antigens immunology
Dexamethasone pharmacology
Ear parasitology
Enzyme-Linked Immunosorbent Assay
Female
Glucocorticoids pharmacology
Humans
Immunity, Humoral drug effects
Immunoglobulin E blood
Immunoglobulin E immunology
Immunoglobulin G blood
Immunoglobulin G immunology
Recombinant Proteins immunology
Scabies drug therapy
Scabies immunology
Skin drug effects
Skin immunology
Skin parasitology
Swine
Swine Diseases drug therapy
Swine Diseases immunology
Time Factors
Immunoglobulin E biosynthesis
Immunoglobulin G biosynthesis
Sarcoptes scabiei immunology
Scabies diagnosis
Scabies veterinary
Swine Diseases diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 8
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 23762351
- Full Text :
- https://doi.org/10.1371/journal.pone.0065354