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Disordered control of intestinal sweet taste receptor expression and glucose absorption in type 2 diabetes.
- Source :
-
Diabetes [Diabetes] 2013 Oct; Vol. 62 (10), pp. 3532-41. Date of Electronic Publication: 2013 Jun 12. - Publication Year :
- 2013
-
Abstract
- We previously established that the intestinal sweet taste receptors (STRs), T1R2 and T1R3, were expressed in distinct epithelial cells in the human proximal intestine and that their transcript levels varied with glycemic status in patients with type 2 diabetes. Here we determined whether STR expression was 1) acutely regulated by changes in luminal and systemic glucose levels, 2) disordered in type 2 diabetes, and 3) linked to glucose absorption. Fourteen healthy subjects and 13 patients with type 2 diabetes were studied twice, at euglycemia (5.2 ± 0.2 mmol/L) or hyperglycemia (12.3 ± 0.2 mmol/L). Endoscopic biopsy specimens were collected from the duodenum at baseline and after a 30-min intraduodenal glucose infusion of 30 g/150 mL water plus 3 g 3-O-methylglucose (3-OMG). STR transcripts were quantified by RT-PCR, and plasma was assayed for 3-OMG concentration. Intestinal STR transcript levels at baseline were unaffected by acute variations in glycemia in healthy subjects and in type 2 diabetic patients. T1R2 transcript levels increased after luminal glucose infusion in both groups during euglycemia (+5.8 × 10(4) and +5.8 × 10(4) copies, respectively) but decreased in healthy subjects during hyperglycemia (-1.4 × 10(4) copies). T1R2 levels increased significantly in type 2 diabetic patients under the same conditions (+6.9 × 10(5) copies). Plasma 3-OMG concentrations were significantly higher in type 2 diabetic patients than in healthy control subjects during acute hyperglycemia. Intestinal T1R2 expression is reciprocally regulated by luminal glucose in health according to glycemic status but is disordered in type 2 diabetes during acute hyperglycemia. This defect may enhance glucose absorption in type 2 diabetic patients and exacerbate postprandial hyperglycemia.
- Subjects :
- 3-O-Methylglucose metabolism
Blood Glucose metabolism
Cross-Over Studies
Diabetes Mellitus, Type 2 metabolism
Epithelial Cells metabolism
Fasting
Female
Glucose Clamp Technique
Humans
Hyperglycemia metabolism
Intestinal Absorption
Intestines pathology
Male
C-Peptide metabolism
Diabetes Mellitus, Type 2 physiopathology
Glycated Hemoglobin metabolism
Hyperglycemia physiopathology
Intestinal Mucosa metabolism
Receptors, G-Protein-Coupled metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1939-327X
- Volume :
- 62
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Diabetes
- Publication Type :
- Academic Journal
- Accession number :
- 23761104
- Full Text :
- https://doi.org/10.2337/db13-0581