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Microorganisms in cryopreserved semen and culture media used in the in vitro production (IVP) of bovine embryos identified by matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS).
- Source :
-
Theriogenology [Theriogenology] 2013 Sep 01; Vol. 80 (4), pp. 337-45. Date of Electronic Publication: 2013 Jun 10. - Publication Year :
- 2013
-
Abstract
- Commercial cattle breeders produce their own herd offspring for the dairy and beef market using artificial insemination. The procedure involves sanitary risks associated with the collection and commercialization of the germplasm, and the in vitro production and transfer of the bovine embryos must be monitored by strict health surveillance. To avoid the spreading of infectious diseases, one must rely on using controlled and monitored germplasm, media, and reagents that are guaranteed free of pathogens. In this article, we investigated the use of a new mass spectrometric approach for fast and accurate identification of bacteria and fungi in bovine semen and in culture media employed in the embryo in vitro production process. The microorganisms isolated from samples obtained in a commercial bovine embryo IVP setting were identified in a few minutes by their conserved peptide/protein profile, obtained applying matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS), matched against a commercial database. The successful microorganisms MS identification has been confirmed by DNA amplification and sequencing. Therefore, the MS technique seems to offer a powerful tool for rapid and accurate microorganism identification in semen and culture media samples.<br /> (Copyright © 2013 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Bacteria isolation & purification
Cryopreservation standards
Culture Media adverse effects
Culture Media analysis
Culture Media standards
Female
Fungi isolation & purification
In Vitro Oocyte Maturation Techniques standards
In Vitro Oocyte Maturation Techniques veterinary
Male
Semen Analysis standards
Semen Preservation adverse effects
Semen Preservation standards
Cattle embryology
Cattle microbiology
Cattle physiology
Cryopreservation veterinary
Embryo, Mammalian microbiology
Semen microbiology
Semen Preservation veterinary
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3231
- Volume :
- 80
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Theriogenology
- Publication Type :
- Academic Journal
- Accession number :
- 23756041
- Full Text :
- https://doi.org/10.1016/j.theriogenology.2013.04.020