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Effects of chemokine (C-C motif) ligand 1 on microglial function.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2013 Jul 05; Vol. 436 (3), pp. 455-61. Date of Electronic Publication: 2013 Jun 06. - Publication Year :
- 2013
-
Abstract
- Microglia, which constitute the resident macrophages of the central nervous system (CNS), are generally considered as the primary immune cells in the brain and spinal cord. Microglial cells respond to various factors which are produced following nerve injury of multiple aetiologies and contribute to the development of neuronal disease. Chemokine (C-C motif) ligand 1 (CCL-1), a well-characterized chemokine secreted by activated T cells, has been shown to play an important role in neuropathic pain induced by nerve injury and is also produced in various cell types in the CNS, especially in dorsal root ganglia (DRG). However, the role of CCL-1 in the CNS and the effects on microglia remains unclear. Here we showed the multiple effects of CCL-1 on microglia. We first showed that CCR-8, a specific receptor for CCL-1, was expressed on primary cultured microglia, as well as on astrocytes and neurons, and was upregulated in the presence of CCL-1. CCL-1 at concentration of 1 ng/ml induced chemotaxis, increased motility at a higher concentration (100 ng/ml), and increased proliferation and phagocytosis of cultured microglia. CCL-1 also activated microglia morphologically, promoted mRNA levels for brain-derived neurotrophic factor (BDNF) and IL-6, and increased the release of nitrite from microglia. These indicate that CCL-1 has a role as a mediator in neuron-glia interaction, which may contribute to the development of neurological diseases, especially in neuropathic pain.<br /> (Copyright © 2013 The Authors. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Astrocytes drug effects
Astrocytes metabolism
Brain-Derived Neurotrophic Factor genetics
Brain-Derived Neurotrophic Factor metabolism
Cell Count
Cell Proliferation
Cell Shape drug effects
Cells, Cultured
Chemokine CCL1 pharmacology
Chemotaxis
Interleukin-6 genetics
Interleukin-6 metabolism
Ki-67 Antigen metabolism
Mice
Microglia drug effects
Neurons drug effects
Neurons metabolism
Nitrites metabolism
Phagocytosis
Primary Cell Culture
RNA, Messenger genetics
RNA, Messenger metabolism
Chemokine CCL1 metabolism
Gene Expression Regulation
Microglia metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 436
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 23747724
- Full Text :
- https://doi.org/10.1016/j.bbrc.2013.05.126