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Proteomic characterization of Pseudomonas aeruginosa PAO1 inner membrane.

Authors :
Casabona MG
Vandenbrouck Y
Attree I
Couté Y
Source :
Proteomics [Proteomics] 2013 Aug; Vol. 13 (16), pp. 2419-23. Date of Electronic Publication: 2013 Jul 17.
Publication Year :
2013

Abstract

Pseudomonas aeruginosa is a Gram-negative bacterium that can inhabit a wide variety of environments and infect different hosts. Human infections by this microbe are nowadays perceived as a major health problem due notably to its multiresistance. The present data set provides the first description of P. aeruginosa inner membrane proteome. To achieve this, we combined efficient separation of membranes from PAO1 reference strain using discontinuous sucrose gradient centrifugation and MS-based proteomic analysis. A core list of 991 nonredundant proteins was established and analyzed in terms of trans-membrane domains, signal peptide, and lipobox sequence prediction. Furthermore, functional insights into membrane-spanning and membrane-associated protein complexes have been explored. All mass spectrometry data have been deposited in the ProteomeXchange with identifier PXD000107.<br /> (© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Details

Language :
English
ISSN :
1615-9861
Volume :
13
Issue :
16
Database :
MEDLINE
Journal :
Proteomics
Publication Type :
Academic Journal
Accession number :
23744604
Full Text :
https://doi.org/10.1002/pmic.201200565