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Retinoid machinery in distinct neural stem cell populations with different retinoid responsiveness.
- Source :
-
Stem cells and development [Stem Cells Dev] 2013 Oct 15; Vol. 22 (20), pp. 2777-93. Date of Electronic Publication: 2013 Jul 24. - Publication Year :
- 2013
-
Abstract
- Retinoic acid (RA) is present at sites of neurogenesis in both the embryonic and adult brain. While it is widely accepted that RA signaling is involved in the regulation of neural stem cell differentiation, little is known about vitamin A utilization and biosynthesis of active retinoids in the neurogenic niches, or about the details of retinoid metabolism in neural stem cells and differentiating progenies. Here we provide data on retinoid responsiveness and RA production of distinct neural stem cell/neural progenitor populations. In addition, we demonstrate differentiation-related changes in the expression of genes encoding proteins of the retinoid machinery, including components responsible for uptake (Stra6) and storage (Lrat) of vitamin A, transport of retinoids (Rbp4, CrbpI, CrabpI-II), synthesis (Rdh10, Raldh1-4), degradation of RA (Cyp26a1-c1) and RA signaling (Rarα,β,γ, Rxrα,β,γ). We show that both early embryonic neuroectodermal (NE-4C) stem cells and late embryonic or adult derived radial glia like progenitors (RGl cells) are capable to produce bioactive retinoids but respond differently to retinoid signals. However, while neuronal differentiation of RGl cells can not be induced by RA, neuron formation by NE-4C cells is initiated by both RA and RA-precursors (retinol or retinyl acetate). The data indicate that endogenous RA production, at least in some neural stem cell populations, may result in autocrine regulation of neuronal differentiation.
- Subjects :
- Adult Stem Cells cytology
Alcohol Oxidoreductases genetics
Alcohol Oxidoreductases metabolism
Aldehyde Dehydrogenase 1 Family
Animals
Cell Differentiation
Cell Lineage genetics
Cytochrome P-450 Enzyme System genetics
Cytochrome P-450 Enzyme System metabolism
Embryonic Stem Cells cytology
Gene Expression Regulation, Developmental
Isoenzymes genetics
Isoenzymes metabolism
Membrane Proteins genetics
Membrane Proteins metabolism
Mice
Neural Stem Cells cytology
Neurogenesis genetics
Neuroglia cytology
Neurons cytology
Primary Cell Culture
Receptors, Retinoic Acid genetics
Receptors, Retinoic Acid metabolism
Retinal Dehydrogenase genetics
Retinal Dehydrogenase metabolism
Retinoic Acid 4-Hydroxylase
Retinol-Binding Proteins, Cellular genetics
Retinol-Binding Proteins, Cellular metabolism
Signal Transduction
Adult Stem Cells metabolism
Embryonic Stem Cells metabolism
Neural Stem Cells metabolism
Neuroglia metabolism
Neurons metabolism
Tretinoin metabolism
Vitamin A metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1557-8534
- Volume :
- 22
- Issue :
- 20
- Database :
- MEDLINE
- Journal :
- Stem cells and development
- Publication Type :
- Academic Journal
- Accession number :
- 23734950
- Full Text :
- https://doi.org/10.1089/scd.2012.0422