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Leptin promotes neointima formation and smooth muscle cell proliferation via NADPH oxidase activation and signalling in caveolin-rich microdomains.
- Source :
-
Cardiovascular research [Cardiovasc Res] 2013 Aug 01; Vol. 99 (3), pp. 555-65. Date of Electronic Publication: 2013 May 30. - Publication Year :
- 2013
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Abstract
- Aims: Apolipoprotein E (apoE) may act as a vasculoprotective factor by promoting plasma lipid clearance and cholesterol efflux. Moreover, apoE accumulates at sites of vascular injury and modulates the effect of growth factors on smooth muscle cells (SMCs). Experimental data suggested that hypothalamic apoE expression is reduced in obesity and associated with leptin resistance. In this study, we examined the role of apoE in mediating the effects of leptin on vascular lesion formation.<br />Methods and Results: Leptin was administered to apoE knockout (apoE-/-) mice via osmotic pumps to increase its circulating levels. Morphometric analysis revealed that leptin did not alter neointima formation and failed to increase α-actin- or PCNA-immunopositive SMCs after vascular injury. Similar findings were obtained after analysis of atherosclerotic lesions. Comparison of apoE-/-, wild-type, or LDL receptor-/- mice and functional analyses in aortic SMCs from WT or apoE-/- mice or human arterial SMCs after treatment with small interfering (si)RNA or heparinase revealed that leptin requires the presence of apoE, expressed, secreted and bound to the cell surface, to fully activate leptin receptor signalling and to promote SMC proliferation and neointima formation. Mechanistically, leptin induced the phosphorylation and membrane translocation of caveolin (cav)-1, and apoE down-regulation or caveolae disruption inhibited the leptin-induced p47phox activation, ROS formation and SMC proliferation. Finally, leptin failed to increase neointima formation in mice lacking cav-1.<br />Conclusion: Our findings suggest that apoE mediates the effects of leptin on vascular lesion formation by stabilizing cav-1-enriched cell membrane microdomains in SMCs, thus allowing NADPH oxidase assembly and ROS-mediated mitogenic signalling.
- Subjects :
- Animals
Apolipoproteins E deficiency
Apolipoproteins E genetics
Caveolin 1 deficiency
Caveolin 1 genetics
Cell Proliferation
Cells, Cultured
Humans
Male
Membrane Microdomains metabolism
Mice
Mice, Inbred C57BL
Mice, Knockout
Neointima pathology
RNA, Small Interfering genetics
Reactive Oxygen Species metabolism
Receptors, LDL deficiency
Receptors, LDL genetics
Signal Transduction
Apolipoproteins E metabolism
Caveolin 1 metabolism
Leptin metabolism
Myocytes, Smooth Muscle metabolism
Myocytes, Smooth Muscle pathology
NADPH Oxidases metabolism
Neointima etiology
Neointima metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1755-3245
- Volume :
- 99
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Cardiovascular research
- Publication Type :
- Academic Journal
- Accession number :
- 23723060
- Full Text :
- https://doi.org/10.1093/cvr/cvt126