Back to Search
Start Over
2-diethylaminoethyl-dextran methyl methacrylate copolymer nonviral vector: still a long way toward the safety of aerosol gene therapy.
- Source :
-
Gene therapy [Gene Ther] 2013 Oct; Vol. 20 (10), pp. 1022-8. Date of Electronic Publication: 2013 May 30. - Publication Year :
- 2013
-
Abstract
- Revealing the lung tumor genome has directed the current treatment strategies toward targeted therapy. First line treatments targeting the genome of lung tumor cells have been approved and are on the market. However, they are limited by the small number of patients with the current investigated genetic mutations. Novel treatment administration modalities have been also investigated in an effort to increase the local drug deposition and disease control. In the current study, we investigated the safety of the new nonviral vector 2-diethylaminoethyl-dextran methyl methacrylate copolymer (DDMC; Ryujyu Science), which belongs to the 2-diethylaminoethyl-dextran family by aerosol administration. Thirty male BALBC mice, 2 month old, were included and divided into three groups. However, pathological findings indicated severe emphysema within three aerosol sessions. In addition, the CytoViva technique was applied for the first time to display the nonviral particles within the pulmonary tissue and emphysema lesions, and a spectral library of the nonviral vector was also established. Although our results in BALBC mice prevented us from further investigation of the DDMC nonviral vector as a vehicle for gene therapy, further investigation in animals with larger airways is warranted to properly evaluate the safety of the vector.
- Subjects :
- Administration, Inhalation
Animals
DEAE-Dextran administration & dosage
Lung Neoplasms therapy
Male
Methylmethacrylate administration & dosage
Mice
Mice, Inbred BALB C
Random Allocation
DEAE-Dextran toxicity
Emphysema chemically induced
Genetic Therapy adverse effects
Genetic Therapy methods
Lung pathology
Methylmethacrylate toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 1476-5462
- Volume :
- 20
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Gene therapy
- Publication Type :
- Academic Journal
- Accession number :
- 23719068
- Full Text :
- https://doi.org/10.1038/gt.2013.27