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CARMA3 overexpression accelerates cell proliferation and inhibits paclitaxel-induced apoptosis through NF-κB regulation in breast cancer cells.
- Source :
-
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine [Tumour Biol] 2013 Oct; Vol. 34 (5), pp. 3041-7. Date of Electronic Publication: 2013 May 25. - Publication Year :
- 2013
-
Abstract
- CARMA3 was recently reported to be overexpressed in several cancers and associated with malignant behavior of cancer cells. However, the expression pattern and biological roles of CARMA3 in breast cancer have not been reported. In the present study, we found that CARMA3 was overexpressed in 41.9 % of breast cancer specimens. Significant association was observed between CARMA3 overexpression and TNM stage (p = 0.0223), tumor size (p = 0.0227), and ErbB-2 status (p = 0.0049). Furthermore, knockdown of CARMA3 expression in MDA-MB-435 cells with high endogenous expression decreased cell proliferation and sensitized cell to paclitaxel-induced apoptosis, while overexpression of CARMA3 in MDA-MB-231 cell line promoted cell proliferation and inhibited apoptosis. Further analysis showed that CARMA3 depletion downregulated, and its overexpression upregulated cyclin D1, Bcl-2, and p-IκB levels. In conclusion, our study demonstrated that CARMA3 is overexpressed in breast cancers. CARMA3 facilitates proliferation and inhibits apoptosis through nuclear factor-kappaB signaling.
- Subjects :
- Breast Neoplasms pathology
CARD Signaling Adaptor Proteins genetics
Case-Control Studies
Cell Line, Tumor
Cell Proliferation
Cyclin D1 metabolism
Female
Gene Expression
Humans
Mammary Glands, Human metabolism
Mammary Glands, Human pathology
Middle Aged
Proto-Oncogene Proteins c-bcl-2 metabolism
Signal Transduction
Antineoplastic Agents, Phytogenic pharmacology
Apoptosis drug effects
Breast Neoplasms metabolism
CARD Signaling Adaptor Proteins metabolism
NF-kappa B metabolism
Paclitaxel pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1423-0380
- Volume :
- 34
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 23708960
- Full Text :
- https://doi.org/10.1007/s13277-013-0869-x