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The incorporation of extracellular matrix proteins in protein polymer hydrogels to improve encapsulated beta-cell function.
- Source :
-
Annals of clinical and laboratory science [Ann Clin Lab Sci] 2013 Spring; Vol. 43 (2), pp. 111-21. - Publication Year :
- 2013
-
Abstract
- Biomaterial encapsulation of islets has been proposed to improve the long-term success of islet transplantation by recreating a suitable microenvironment and enhancing cell-matrix interactions that affect cellular function. Protein polymer hydrogels previously showed promise as a biocompatible scaffold by maintaining high cell viability. Here, enzymatically-crosslinked protein polymers were used to investigate the effects of varying scaffold properties and of introducing ECM proteins on the viability and function of encapsulated MIN6 β-cells. Chemical and mechanical properties of the hydrogel were modified by altering the protein concentrations while collagen IV, fibronectin, and laminin were incorporated to reestablish cell-matrix interactions lost during cell isolation. Rheology indicated all hydrogels formed quickly, resulting in robust, elastic hydrogels with Young's moduli similar to soft tissue. All hydrogels tested supported both high MIN6 β-cell viability and function and have the potential to serve as an encapsulation platform for islet cell delivery in vivo.
- Subjects :
- Adenosine Triphosphate metabolism
Amino Acid Sequence
Animals
Biocompatible Materials metabolism
Cell Line
Cell Survival drug effects
Chromatography, Affinity
Collagen
Fibronectins
Insulin-Secreting Cells drug effects
Laminin
Mice
Molecular Sequence Data
Rheology
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Cellular Microenvironment physiology
Extracellular Matrix Proteins pharmacology
Hydrogels metabolism
Insulin-Secreting Cells physiology
Islets of Langerhans Transplantation methods
Polymers pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1550-8080
- Volume :
- 43
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Annals of clinical and laboratory science
- Publication Type :
- Academic Journal
- Accession number :
- 23694784