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Characterization of the T cell response in allergic contact dermatitis caused by corticosteroids.
- Source :
-
Contact dermatitis [Contact Dermatitis] 2013 Jun; Vol. 68 (6), pp. 357-68. - Publication Year :
- 2013
-
Abstract
- Background: Delayed allergic hypersensitivity reactions have classically been described as type IV reactions, which are caused by T cells; however, the respective roles of CD4(+) and CD8(+) cells are yet to be defined. A central role for CD8(+) cytotoxic T cells as effector cells has been suggested.<br />Objectives: To determine the type of T cell involved in corticosteroid allergy.<br />Methods: We analysed the kinetics of T cell recruitment and the cytokine production profile in positive patch tests of 27 corticosteroid-sensitized patients, as compared with control sites and control subjects. Skin biopsies, collected at 8, 24 and 48 hr following drug application, were embedded in paraffin for histological and immunohistological staining, and, in some cases, also deep-frozen for gene expression analyses.<br />Results: CD3(+) T cells were rapidly recruited in concert with the positivity of the patch test sites. High levels of interleukin (IL)-4, IL-5 and, to a lesser extent, interferon-γ suggested that both Th2 and Th1 cytokines were implicated. IL-4 was also produced by γδ T cell receptor (TCR) lymphocytes.<br />Conclusions: This study showed that, in allergic contact dermatitis caused by corticosteroids, the inflammatory infiltrate is composed of CD3(+) T cells with a predominant Th2 cytokine profile, among which IL-4 is also produced by γδ TCR lymphocytes.<br /> (© 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Subjects :
- Adult
Aged
Biomarkers metabolism
Biopsy
CD3 Complex metabolism
Case-Control Studies
Dermatitis, Allergic Contact diagnosis
Dermatitis, Allergic Contact immunology
Drug Eruptions diagnosis
Drug Eruptions immunology
Female
Flow Cytometry
Humans
Hydrocortisone adverse effects
Immunohistochemistry
Interferon-gamma metabolism
Interleukin-4 metabolism
Interleukin-5 metabolism
Linear Models
Logistic Models
Male
Middle Aged
Patch Tests
Receptors, Antigen, T-Cell, gamma-delta metabolism
Reverse Transcriptase Polymerase Chain Reaction
Skin immunology
Skin pathology
Budesonide adverse effects
Dermatitis, Allergic Contact etiology
Drug Eruptions etiology
Glucocorticoids adverse effects
Hydrocortisone analogs & derivatives
T-Lymphocytes metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1600-0536
- Volume :
- 68
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Contact dermatitis
- Publication Type :
- Academic Journal
- Accession number :
- 23692036
- Full Text :
- https://doi.org/10.1111/cod.12040