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Revised relative potency values for PCDDs, PCDFs, and non-ortho-substituted PCBs for the optimized H4IIE-luc in vitro bioassay.
- Source :
-
Environmental science and pollution research international [Environ Sci Pollut Res Int] 2013 Dec; Vol. 20 (12), pp. 8590-9. Date of Electronic Publication: 2013 May 21. - Publication Year :
- 2013
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Abstract
- While the World Health Organization 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) equivalency factors are useful estimates of relative potencies of mixtures when conducting risk assessments, they are not useful when comparing the results of bioassays such as the H4IIE-luc to concentrations of TCDD equivalents calculated from instrumental analyses. Since there are thousands of dioxin-like compounds (DLCs), one use of screening assays is to determine if all of the aryl hydrocarbon receptor (AhR) active DLCs in a mixture have been accounted for in instrumental analyses. For this purpose, bioassay-specific relative potency (ReP) values are needed. RePs of 21 polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans, and dioxin-like polychlorinated biphenyls that exhibit effects mediated through the AhR were determined by use of the H4IIE-luc assay. Different values of RePs are derived, depending on the statistical, curve-fitting methods used to derive them from the dose-response relationships. Here, we discuss the various methods for deriving RePs from in vitro data and their assumptions and effects on values of RePs. Full dose-response curves of 2,3,7,8-TCDD and other representative DLCs were used to estimate effective concentrations at multiple points (e.g., EC20-50-80), which were then used to estimate ReP of each DLC to 2,3,7,8-TCDD.
- Subjects :
- Animals
Benzofurans standards
Biological Assay methods
Dibenzofurans, Polychlorinated
Dioxins standards
Dioxins toxicity
Dose-Response Relationship, Drug
Humans
Polychlorinated Biphenyls standards
Polychlorinated Dibenzodioxins standards
Polychlorinated Dibenzodioxins toxicity
Receptors, Aryl Hydrocarbon
Benzofurans toxicity
Biological Assay standards
Polychlorinated Biphenyls toxicity
Polychlorinated Dibenzodioxins analogs & derivatives
Subjects
Details
- Language :
- English
- ISSN :
- 1614-7499
- Volume :
- 20
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Environmental science and pollution research international
- Publication Type :
- Academic Journal
- Accession number :
- 23690076
- Full Text :
- https://doi.org/10.1007/s11356-013-1770-2