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Regulation of the swarming inhibitor disA in Proteus mirabilis.
- Source :
-
Journal of bacteriology [J Bacteriol] 2013 Jul; Vol. 195 (14), pp. 3237-43. Date of Electronic Publication: 2013 May 17. - Publication Year :
- 2013
-
Abstract
- The disA gene encodes a putative amino acid decarboxylase that inhibits swarming in Proteus mirabilis. 5' rapid amplification of cDNA ends (RACE) and deletion analysis were used to identify the disA promoter. The use of a disA-lacZ fusion indicated that FlhD(4)C(2), the class I flagellar master regulator, did not have a role in disA regulation. The putative product of DisA, phenethylamine, was able to inhibit disA expression, indicating that a negative regulatory feedback loop was present. Transposon mutagenesis was used to identify regulators of disA and revealed that umoB (igaA) was a negative regulator of disA. Our data demonstrate that the regulation of disA by UmoB is mediated through the Rcs phosphorelay.
- Subjects :
- Artificial Gene Fusion
DNA Transposable Elements
Gene Expression Regulation, Enzymologic
Genes, Reporter
Mutagenesis, Insertional
Phenethylamines metabolism
Promoter Regions, Genetic
Proteus mirabilis metabolism
beta-Galactosidase analysis
beta-Galactosidase genetics
Bacterial Proteins genetics
Bacterial Proteins metabolism
Gene Expression Regulation, Bacterial
Locomotion
Proteus mirabilis genetics
Proteus mirabilis physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1098-5530
- Volume :
- 195
- Issue :
- 14
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 23687266
- Full Text :
- https://doi.org/10.1128/JB.00039-13