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Discarded human kidneys as a source of ECM scaffold for kidney regeneration technologies.
- Source :
-
Biomaterials [Biomaterials] 2013 Aug; Vol. 34 (24), pp. 5915-25. Date of Electronic Publication: 2013 May 13. - Publication Year :
- 2013
-
Abstract
- In the United States, more than 2600 kidneys are discarded annually, from the total number of kidneys procured for transplant. We hypothesized that this organ pool may be used as a platform for renal bioengineering and regeneration research. We previously showed that decellularization of porcine kidneys yields renal extracellular matrix (ECM) scaffolds that maintain their basic components, support cell growth and welfare in vitro and in vivo, and show an intact vasculature that, when such scaffolds are implanted in vivo, is able to sustain physiological blood pressure. The purpose of the current study was to test if the same strategy can be applied to discarded human kidneys in order to obtain human renal ECM scaffolds. The results show that the sodium dodecylsulfate-based decellularization protocol completely cleared the cellular compartment in these kidneys, while the innate ECM framework retained its architecture and biochemical properties. Samples of human renal ECM scaffolds stimulated angiogenesis in a chick chorioallantoic membrane assay. Importantly, the innate vascular network in the human renal ECM scaffolds retained its compliance. Collectively, these results indicate that discarded human kidneys are a suitable source of renal scaffolds and their use for tissue engineering applications may be more clinically applicable than kidneys derived from animals.<br /> (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Antigens metabolism
Biomarkers metabolism
Chickens
Extracellular Matrix ultrastructure
Humans
Immunohistochemistry
Kidney blood supply
Kidney cytology
Kidney ultrastructure
Kidney Transplantation
Neovascularization, Physiologic
Pressure
Extracellular Matrix metabolism
Kidney physiology
Regeneration physiology
Regenerative Medicine methods
Tissue Scaffolds chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1878-5905
- Volume :
- 34
- Issue :
- 24
- Database :
- MEDLINE
- Journal :
- Biomaterials
- Publication Type :
- Academic Journal
- Accession number :
- 23680364
- Full Text :
- https://doi.org/10.1016/j.biomaterials.2013.04.033