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[Experimental study on human amniotic membrane for repairing tendon sheath defect].
- Source :
-
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery [Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi] 2013 Mar; Vol. 27 (3), pp. 335-9. - Publication Year :
- 2013
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Abstract
- Objective: To investigate the feasibility and effect of human amniotic membrane in prevention of tendon adhension after tendon sheat defect repair.<br />Methods: The amniotic membrane in size of 1.5 cm x 1.0 cm was harvested from human placenta which was voluntary donated from maternal after cesarean. Forty healthy male Leghorn chicken (aged 3-6 months) were selected, weighing (1.86 +/- 0.04) kg. The model of flexor digitorum profundus tendon and tendon sheath defects was established at the third toe. After repair of the flexor digitorum profundus tendon, the human amniotic membrane was used to repair the tendon sheath defect in the right foot (group A), but tendon sheath defect was not repaired in the left foot (group B) . At 1, 2, 4, and 6 weeks after operation, the gross and histological observations were done; the degree of tendon adhesions was graded according to Tang's tendon adhesion general observation grading standards; and the biomechanical properties (tendon slip length and total flexion angle) were tested.<br />Results: All animals survived after operation and incisions healed. Gross and histological observations showed that the new tendon sheath formed with time passing after operation in groups A and B; new tendon sheath was more maturer and smoother in group A than in group B. The degree of tendon adhesions in group A was significantly less than that in group B (P < 0.05) at 1 and 6 weeks after operation. The biomechanical test results showed there was no significant difference in the tendon slip length between 2 groups at 1 and 2 weeks after operation (P > 0.05), but the tendon slip length of group A was significantly longer than that of group B at 4 and 6 weeks after operation (P < 0.05). The total flexion angle of group A was significantly smaller than that of group B at 1, 2, 4, and 6 weeks after operation (P < 0.05).<br />Conclusion: It is effective in the prevention of tendon adhesion to use the amniotic membrane for repairing the tendon sheath defect, which is beneficial to recovery of the tendon sliding function.
- Subjects :
- Animals
Biomechanical Phenomena
Chickens
Feasibility Studies
Female
Humans
Male
Recovery of Function
Tendons pathology
Time Factors
Tissue Adhesions pathology
Toes pathology
Wound Healing
Amnion transplantation
Tendon Injuries surgery
Tendons surgery
Tissue Adhesions prevention & control
Toes surgery
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1002-1892
- Volume :
- 27
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery
- Publication Type :
- Academic Journal
- Accession number :
- 23672137