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Effects of carboxymethylpachymaran on signal molecules in chicken immunocytes.
- Source :
-
International journal of biological macromolecules [Int J Biol Macromol] 2013 Aug; Vol. 59, pp. 357-62. Date of Electronic Publication: 2013 May 07. - Publication Year :
- 2013
-
Abstract
- The study was carried out to investigate the immunomodulation mechanism of carboxymethylpachymaran (CMP). Chicken splenic lymphocytes were cultured in medium alone or with CMP at the final concentration of 50mg/L, 100mg/L, 200mg/L or 400mg/L in vitro for 4h, 8h, 12h or 24h, respectively. The supernatants at different culture periods were analyzed for changes in levels of 6-keto-prostaglandin F1α (6-keto-PGF1α), thromboxane B2 (TXB2) and nitric oxide (NO). The cells were collected to determine contents of oxidized glutathione (GSSG), reduced glutathione (GSH), cyclic AMP (cAMP) and cyclic GMP (cGMP). The results showed that CMP increase the values of NO, 6-keto-PGF1α, TXB2, and the ratio of 6-keto-PGF1α to TXB2 in supernatants. The contents of intracellular GSH, cAMP, cGMP and the ratio of cAMP to cGMP were increased in the cells treated with CMP. The results suggested that CMP enhanced immune functions by increasing the contents of GSH and by regulating arachidonic acid signal transduction systems in chicken splenic lymphocytes. The signal pathway of NO-cGMP plays an important role in CMP-induced activation of chicken splenic lymphocytes.<br /> (Copyright © 2013 Elsevier B.V. All rights reserved.)
- Subjects :
- 6-Ketoprostaglandin F1 alpha biosynthesis
6-Ketoprostaglandin F1 alpha metabolism
Animals
Cells, Cultured
Chickens
Cyclic AMP metabolism
Cyclic GMP metabolism
Dose-Response Relationship, Immunologic
Glutathione metabolism
Glutathione Disulfide metabolism
Lymphocyte Activation
Lymphocytes cytology
Lymphocytes immunology
Nitric Oxide biosynthesis
Nitric Oxide metabolism
Spleen cytology
Spleen immunology
Thromboxane B2 biosynthesis
Thromboxane B2 metabolism
6-Ketoprostaglandin F1 alpha immunology
Glucans pharmacology
Lymphocytes drug effects
Nitric Oxide immunology
Signal Transduction drug effects
Spleen drug effects
Thromboxane B2 immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0003
- Volume :
- 59
- Database :
- MEDLINE
- Journal :
- International journal of biological macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 23664932
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2013.04.071