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High interleukin-10 expression within the central nervous system may be important for initiation of recovery of Dark Agouti rats from experimental autoimmune encephalomyelitis.
- Source :
-
Immunobiology [Immunobiology] 2013 Sep; Vol. 218 (9), pp. 1192-9. Date of Electronic Publication: 2013 Apr 12. - Publication Year :
- 2013
-
Abstract
- Dark Agouti (DA) rats are highly susceptible to induction of experimental autoimmune encephalomyelitis (EAE), still they completely recover from the disease. Here, we were interested to determine contribution of major anti-inflammatory cytokines transforming growth factor (TGF)-β and interleukin (IL)-10 to the recovery of DA rats from EAE. To that extent we determined CNS expression of these cytokines in DA rats at different phases of EAE and compared data to those obtained in EAE-resistant Albino Oxford (AO) rats. Higher expression of TGF-β was persistently observed in the CNS of AO rats, even if rats were not immunized. This implied that high TGF-β within the CNS is important for resistance of AO rats to EAE induction. On the contrary, IL-10 expression was consistently higher in DA than in AO rats and it culminated at the peak of EAE. Methylprednisolone suppressed EAE and expression of IL-10 in spinal cord homogenates, while IL-10 was increased in CNS-infiltrating immune cells. This implied that IL-10 might have a significant role in recovery of DA rats from the disease. Thus, we next explored effects of IL-10 on astrocytes, glial cells that largely contribute to control of CNS inflammation. IL-10 stimulated astrocytic expression of an important regulator of neuroinflammation, CXCL12. Thus, IL-10 might contribute to recovery of DA rats from EAE through induction of CXCL12 expression in astrocytes.<br /> (Copyright © 2013 Elsevier GmbH. All rights reserved.)
- Subjects :
- Animals
Cells, Cultured
Chemokine CXCL12 genetics
Chemokine CXCL12 metabolism
Disease Progression
Gene Expression Regulation drug effects
Gene Expression Regulation immunology
Interleukin-10 genetics
Methylprednisolone administration & dosage
Rats
Rats, Inbred Strains
Transforming Growth Factor beta genetics
Astrocytes immunology
Convalescence
Encephalomyelitis, Autoimmune, Experimental immunology
Interleukin-10 metabolism
Spinal Cord immunology
Transforming Growth Factor beta metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1878-3279
- Volume :
- 218
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Immunobiology
- Publication Type :
- Academic Journal
- Accession number :
- 23664544
- Full Text :
- https://doi.org/10.1016/j.imbio.2013.04.004