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The succinoglycan endoglycanase encoded by exoK is required for efficient symbiosis of Sinorhizobium meliloti 1021 with the host plants Medicago truncatula and Medicago sativa (Alfalfa).
- Source :
-
Molecular plant-microbe interactions : MPMI [Mol Plant Microbe Interact] 2013 Sep; Vol. 26 (9), pp. 1089-105. - Publication Year :
- 2013
-
Abstract
- The acidic polysaccharide succinoglycan produced by the nitrogen-fixing rhizobial symbiont Sinorhizobium meliloti 1021 is required for this bacterium to invade the host plant Medicago truncatula and to efficiently invade the host plant M. sativa (alfalfa). The β-glucanase enzyme encoded by exoK has previously been demonstrated to cleave succinoglycan and participate in producing the low molecular weight form of this polysaccharide. Here, we show that exoK is required for efficient S. meliloti invasion of both M. truncatula and alfalfa. Deletion mutants of exoK have a substantial reduction in symbiotic productivity on both of these plant hosts. Insertion mutants of exoK have an even less productive symbiosis than the deletion mutants with the host M. truncatula that is caused by a secondary effect of the insertion itself, and may be due to a polar effect on the expression of the downstream exoLAMON genes.
- Subjects :
- Bacterial Proteins genetics
Bacterial Proteins metabolism
Biomass
Genetic Complementation Test
Glycoside Hydrolases metabolism
Mutation
Nitrogen Fixation
Phenotype
Plant Root Nodulation
Plant Roots microbiology
Plant Shoots microbiology
Polysaccharides, Bacterial metabolism
Recombinant Fusion Proteins
Sinorhizobium meliloti genetics
Sinorhizobium meliloti physiology
Glycoside Hydrolases genetics
Medicago sativa microbiology
Medicago truncatula microbiology
Sinorhizobium meliloti enzymology
Symbiosis
Subjects
Details
- Language :
- English
- ISSN :
- 0894-0282
- Volume :
- 26
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Molecular plant-microbe interactions : MPMI
- Publication Type :
- Academic Journal
- Accession number :
- 23656330
- Full Text :
- https://doi.org/10.1094/MPMI-03-13-0087-R