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Glucocorticoid exerts its non-genomic effect on IPSC by activation of a phospholipase C-dependent pathway in prefrontal cortex of rats.
- Source :
-
The Journal of physiology [J Physiol] 2013 Jul 01; Vol. 591 (13), pp. 3341-53. Date of Electronic Publication: 2013 May 07. - Publication Year :
- 2013
-
Abstract
- In response to stressor, the brain activates a comprehensive stress system. Among others, this stress system causes release of glucocorticoids that also feed back to the brain. Glucocorticoids affect brain function by activation of both delayed, genomic and rapid, non-genomic mechanisms in rodents. Here we report that application of the potent glucocorticoid receptor agonist dexamethasone (DEX) caused a rapid increase of spontaneous and miniature inhibitory postsynaptic currents (IPSCs) and elicited intermittent burst activities through a non-genomic pathway, involving membrane-located receptors. The onset of the rapid effect in prefrontal cortex (PFC, <15 min) was much slower than in hippocampus (<5 min). The intermittent burst activities were abolished in the presence of TTX. Furthermore, the nitric oxide (NO) pathway was present and endogenously activated in PFC. Part of the rapid DEX effect in PFC remained after blocking NO-sensitive guanylyl cyclase that was due to activation of a phospholipase C-diacylglycerol-dependent signalling pathway. Thus, our data demonstrated that glucocorticoids could rapidly enhance IPSCs and evoke burst activities by activation of at least two different signalling pathways in hippocampus and PFC of rats.
- Subjects :
- Animals
GABAergic Neurons drug effects
GABAergic Neurons physiology
Hippocampus physiology
In Vitro Techniques
Inhibitory Postsynaptic Potentials drug effects
Male
Nitric Oxide physiology
Prefrontal Cortex physiology
Rats
Rats, Sprague-Dawley
Receptors, Glucocorticoid agonists
Dexamethasone pharmacology
Glucocorticoids pharmacology
Hippocampus drug effects
Prefrontal Cortex drug effects
Type C Phospholipases physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1469-7793
- Volume :
- 591
- Issue :
- 13
- Database :
- MEDLINE
- Journal :
- The Journal of physiology
- Publication Type :
- Academic Journal
- Accession number :
- 23652592
- Full Text :
- https://doi.org/10.1113/jphysiol.2013.254961