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Xenoestrogenic and dioxin-like activity in blood of East Greenland polar bears (Ursus maritimus).
- Source :
-
Chemosphere [Chemosphere] 2013 Jul; Vol. 92 (5), pp. 583-91. Date of Electronic Publication: 2013 May 03. - Publication Year :
- 2013
-
Abstract
- The aims of the project were to (i) extract the lipophilic persistent organic pollutants (POPs) from the blood of 99 East Greenland polar bears and assess the combined mixture effect on the estrogen receptor (ER) and the aryl hydrocarbon receptor (AhR) mediated transactivity; (ii) To evaluate whether the receptor transactivities were associated with selected POP markers, and (iii) compare the receptor transactivities in polar bears with earlier studies on Greenlandic Inuit. Lipophilic POPs were extracted using a combination of solid-phase extraction (SPE) and high performance liquid chromatography (HPLC). ER mediated transactivity was determined using the ER luciferase reporter MVLN cell assay. The extracts were tested alone (XER) and together with 17β-estradiol (E2) as a physiological mimic (XERcomp). Dioxins and dioxin-like (DL) compounds were extracted by a combination of SPE and the Supelco Dioxin Prep System®. AhR mediated dioxin-like transactivity was determined using the AhR luciferase reporter Hepa 1.12cR cell assay. Agonistic ER transactivity was elicited by 19% of the samples, and a further increased E2 induced ER response was found for 52%, whereas 17% antagonized the E2 induced ER response. Positive correlations were found in subadult bears between XER and several POP biomarkers. XER and XERcomp correlated positively to each other. A total of 91% of the polar bear blood extracts elicited agonistic AhR transactivity. The AhR-TCDD equivalent (AhR-TEQ) median levels were higher among adult bears compared to subadult bears, but not significantly.<br /> (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Dioxins metabolism
Environmental Pollutants metabolism
Female
Male
Pesticides blood
Pesticides metabolism
Polychlorinated Biphenyls blood
Polychlorinated Biphenyls metabolism
Ursidae metabolism
Dioxins blood
Environmental Pollutants blood
Receptors, Aryl Hydrocarbon metabolism
Receptors, Estrogen metabolism
Ursidae blood
Subjects
Details
- Language :
- English
- ISSN :
- 1879-1298
- Volume :
- 92
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Chemosphere
- Publication Type :
- Academic Journal
- Accession number :
- 23648332
- Full Text :
- https://doi.org/10.1016/j.chemosphere.2013.03.059