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LPS challenge regulates gene expression and tissue localization of a Ciona intestinalis gene through an alternative polyadenylation mechanism.
LPS challenge regulates gene expression and tissue localization of a Ciona intestinalis gene through an alternative polyadenylation mechanism.
- Source :
-
PloS one [PLoS One] 2013 Apr 30; Vol. 8 (4), pp. e63235. Date of Electronic Publication: 2013 Apr 30 (Print Publication: 2013). - Publication Year :
- 2013
-
Abstract
- A subtractive hybridization strategy for the identification of differentially expressed genes was performed between LPS-challenged and naive Ciona intestinalis. This strategy allowed the characterization of two transcripts (Ci8short and Ci8long) generated by the use of two Alternative Polyadenylation sites. The Ci8long transcript contains a protein domain with relevant homology to several components of the Receptor Transporting Protein (RTP) family not present in the Ci8short mRNA. By means of Real Time PCR and Northern Blot, the Ci8short and Ci8long transcripts showed a different pattern of gene expression with the Ci8short mRNA being strongly activated after LPS injection in the pharynx. In situ hybridization analysis demonstrated that the activation of the APA site also influenced the tissue localization of the Ci8short transcript. This analysis showed that the Ci8long mRNA was expressed in hemocytes meanwhile the Ci8short mRNA was highly transcribed also in vessel endothelial cells and in the epithelium of pharynx. These findings demonstrated that regulation of gene expression based on different polyadenylation sites is an ancestral powerful strategy influencing both the level of expression and tissue distribution of alternative transcripts.
- Subjects :
- 3' Untranslated Regions
5' Untranslated Regions
Amino Acid Sequence
Animals
Base Sequence
Ciona intestinalis classification
Ciona intestinalis immunology
Computational Biology
DNA, Complementary chemistry
Gene Order
Lipopolysaccharides immunology
Molecular Sequence Data
Phylogeny
Protein Transport
Proteins chemistry
Proteins genetics
Proteins metabolism
RNA, Messenger genetics
RNA, Messenger metabolism
Sequence Alignment
Ciona intestinalis genetics
Ciona intestinalis metabolism
Gene Expression
Genes
Polyadenylation
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 8
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 23646203
- Full Text :
- https://doi.org/10.1371/journal.pone.0063235