Heparanase-1 activities in the development of laser induced choroidal neovascularization.

Authors :: Tang WQ
Hou BK
Source :: International journal of ophthalmology [Int J Ophthalmol] 2013 Apr 18; Vol. 6 (2), pp. 131-5. Date of Electronic Publication: 2013 Apr 18 (Print Publication: 2013).
Publication Year :: 2013
Abstract: Aim: To investigate the role of heparanase-1 in laser-induced choroidal neovascularization (CNV).<br />Methods: Experimental CNV was induced by krypton laser photocoagulation in 15 male Brown Norway rats. Fundus fluorescein angiography and histopathological examination were performed in observing the CNV development. The expression and distribution of heparanase-1 protein in the laser lesions were determined by immunohistochemistry and western blotting analysis.<br />Results: The success rate of laser induced CNV was approximately 75% on 3-4 weeks after laser photocoagulation. The protein levels of heparanase-1 increased significantly in the retina-choroidal complex of CNV models when compared to normal rat eyes (P<0.01). Immunostaining confirmed strong heparanase-1 expressions in all laser lesions, and it displayed to be highest at the newly formed blood vessels within the fibrovascular complex in the subretinal space.<br />Conclusion: Heparanase-1 is closely involved in the development of laser induced CNV.

Full Text Access

Tools