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Macromolecule extravasation-xenograft size matters: a systematic study using probe-based confocal laser endomicroscopy (pCLE).
- Source :
-
Molecular imaging and biology [Mol Imaging Biol] 2013 Dec; Vol. 15 (6), pp. 693-702. - Publication Year :
- 2013
-
Abstract
- Purpose: Profound changes of the vasculature in tumors critically impact drug delivery and therapy response. We aimed at developing a procedure to monitor morphological and functional parameters of the vasculature in subcutaneous xenograft models commonly applied for therapy testing by using probe-based confocal laser endomicroscopy.<br />Procedures: By monitoring various normal and diseased tissues, we established an experimental and analytical set-up to systematically analyze tracer extravasation from the microvasculature. Application of the approach in two xenograft models (HCT-116 and SW620) was realized consecutively throughout tumor growth.<br />Results: The incidence of dilated vessels increased with xenograft size in both models while macromolecule extravasation and tracer accumulation in the tumor tissue, respectively, was significantly reduced throughout growth. The development of dilated/ultradilated vessels correlated with tracer extravasation only in the HCT-116 but not the SW620 model. The underlying mechanisms are still ambiguous and discussed.<br />Conclusions: Our findings clearly indicate that both xenograft type and size matter for drug delivery and therapy testing.
- Subjects :
- Animals
Female
HCT116 Cells
Humans
Mice
Mice, Inbred C57BL
Muscles blood supply
Muscles pathology
Neoplasms, Experimental
Tongue blood supply
Tongue pathology
Xenograft Model Antitumor Assays
Capillary Permeability
Extravasation of Diagnostic and Therapeutic Materials pathology
Microscopy, Confocal methods
Neovascularization, Pathologic pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1860-2002
- Volume :
- 15
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Molecular imaging and biology
- Publication Type :
- Academic Journal
- Accession number :
- 23632953
- Full Text :
- https://doi.org/10.1007/s11307-013-0641-z