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RNA-Seq reveals expression signatures of genes involved in oxygen transport, protein synthesis, folding, and degradation in response to heat stress in catfish.
- Source :
-
Physiological genomics [Physiol Genomics] 2013 Jun 17; Vol. 45 (12), pp. 462-76. Date of Electronic Publication: 2013 Apr 30. - Publication Year :
- 2013
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Abstract
- Temperature is one of the most prominent abiotic factors affecting ectotherms. Most fish species, as ectotherms, have extraordinary ability to deal with a wide range of temperature changes. While the molecular mechanism underlying temperature adaptation has long been of interest, it is still largely unexplored with fish. Understanding of the fundamental mechanisms conferring tolerance to temperature fluctuations is a topic of increasing interest as temperature may continue to rise as a result of global climate change. Catfish have a wide natural habitat and possess great plasticity in dealing with environmental variations in temperature. However, no studies have been conducted at the transcriptomic level to determine heat stress-induced gene expression. In the present study, we conducted an RNA-Seq analysis to identify heat stress-induced genes in catfish at the transcriptome level. Expression analysis identified a total of 2,260 differentially expressed genes with a cutoff of twofold change. qRT-PCR validation suggested the high reliability of the RNA-Seq results. Gene ontology, enrichment, and pathway analyses were conducted to gain insight into physiological and gene pathways. Specifically, genes involved in oxygen transport, protein folding and degradation, and metabolic process were highly induced, while general protein synthesis was dramatically repressed in response to the lethal temperature stress. This is the first RNA-Seq-based expression study in catfish in response to heat stress. The candidate genes identified should be valuable for further targeted studies on heat tolerance, thereby assisting the development of heat-tolerant catfish lines for aquaculture.
- Subjects :
- Adaptation, Physiological genetics
Animals
Biological Transport genetics
Body Size genetics
Catfishes anatomy & histology
Gills metabolism
Liver metabolism
Molecular Sequence Annotation
Reproducibility of Results
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction genetics
Transcriptome genetics
Catfishes genetics
Gene Expression Profiling
Heat-Shock Response genetics
Oxygen metabolism
Protein Biosynthesis genetics
Protein Folding
Proteolysis
Sequence Analysis, RNA
Subjects
Details
- Language :
- English
- ISSN :
- 1531-2267
- Volume :
- 45
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Physiological genomics
- Publication Type :
- Academic Journal
- Accession number :
- 23632418
- Full Text :
- https://doi.org/10.1152/physiolgenomics.00026.2013