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Biopolymer-based hydrogels as injectable materials for tissue repair scaffolds.
- Source :
-
Biomedical materials (Bristol, England) [Biomed Mater] 2013 Jun; Vol. 8 (3), pp. 035013. Date of Electronic Publication: 2013 Apr 29. - Publication Year :
- 2013
-
Abstract
- The progress in tissue regeneration is strongly dependent on the development of biocompatible materials with properties resembling those of a native tissue. Also, the application of noninvasive methods of delivering the scaffold into the tissue defect is of great importance. In this study we present a group of biopolymer-based materials as potential injectable scaffolds. In contrast to other studies involving collagen neutralization or additional incubation of gel in genipin solution, we propose collagen and collagen-chitosan gels crosslinked in situ with genipin. Since some parameters of the cells should be considered in the microscale, the steady-state fluorescence anisotropy was applied to study the microenvironment of the gels. To our knowledge we are the first to report on microrheological properties, such as gel time and microviscosity, for this group of hydrogels. Rapid gelation at physiological temperatures found makes these materials of special interest in applications requiring gel injectability. Physico-chemical investigation showed the influence of the crosslinking agent concentration and chitosan addition on the crosslinking degree, swelling ratio, gel microviscosity, and the degradation rate. Strong correlation was revealed between the surface wettability and the viability of cultured mesenchymal stem cells. Cytotoxicity studies indicated that the collagen-chitosan hydrogels showed the best biocompatibility.
- Subjects :
- Cell Survival
Chitosan chemistry
Collagen chemistry
Cross-Linking Reagents
Fluorescence Polarization
Humans
Hydrogels administration & dosage
Hydrogels chemistry
Injections
Iridoids
Materials Testing
Mesenchymal Stem Cells cytology
Microscopy, Electron, Scanning
Regeneration
Viscosity
Biopolymers administration & dosage
Biopolymers chemistry
Tissue Engineering methods
Tissue Scaffolds chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1748-605X
- Volume :
- 8
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Biomedical materials (Bristol, England)
- Publication Type :
- Academic Journal
- Accession number :
- 23629621
- Full Text :
- https://doi.org/10.1088/1748-6041/8/3/035013