A convenient method for selective detection of 5-hydroxymethylcytosine and 5-formylcytosine sites in DNA sequences.

Authors :: Mao W
Hu J
Hong T
Xing X
Wang S
Chen X
Zhou X
Source :: Organic & biomolecular chemistry [Org Biomol Chem] 2013 Jun 07; Vol. 11 (21), pp. 3568-72. Date of Electronic Publication: 2013 Apr 30.
Publication Year :: 2013
Abstract: Treatment of DNA containing 5-formylcytosine with hot piperidine produced a cleaved band at the position of 5-formylcytosine in DNA sequences. After oxidation with KRuO4, 5-hydroxymethylcytosine could also be detected using the same method. Using our strategy, we could detect 5-hydroxymethylcytosine and 5-formylcytosine respectively.

Subjects :: 5-Methylcytosine analogs & derivatives
Chromatography, High Pressure Liquid
Cytosine chemistry
Electrophoresis, Gel, Two-Dimensional
Sequence Analysis, DNA
Base Sequence
Combinatorial Chemistry Techniques
Cytosine analogs & derivatives

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