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Expression of WWOX and FHIT is downregulated by exposure to arsenite in human uroepithelial cells.
- Source :
-
Toxicology letters [Toxicol Lett] 2013 Jul 04; Vol. 220 (2), pp. 118-25. Date of Electronic Publication: 2013 Apr 22. - Publication Year :
- 2013
-
Abstract
- Ecological studies in Taiwan, Chile, Argentina, Bangladesh, and Mexico have confirmed significant dose-dependent associations between ingestion of arsenic-contaminated drinking water and the risk of various human malignancies. The FHIT and WWOX genes are active in common fragile sites FRA3B and FRA16D, respectively. Reduced expression of FHIT or WWOX is known to be an early indicator of carcinogen-induced cancers. However, the effect of arsenite on the expressions and molecular mechanisms of these markers is still unclear. The aims of this study were (i) to observe the expression of ATR, WWOX and FHIT proteins in urothelial carcinoma (UC) between endemic and non-endemic areas of blackfoot disease (BFD) by immunohistochemical analyses; (ii) to compare expression of these genes between arsenite-treated SV-HUC-1 human epithelial cells and rat uroepithelial cells; and (iii) to determine the role of DNMT and MEK inhibitors on expressions of WWOX and FHIT in response to arsenite in SV-HUC-1. The experiments revealed that expressions of ATR, WWOX and FHIT in UC significantly differed between BFD areas and non-BFD areas (p=0.003, 0.009 and 0.021, respectively). In fact, the results for the arsenite-treated groups showed that ATR, WWOX and FHIT are downregulated by arsenite in SV-HUC-1. However, the inhibitors suppressed the effects of arsenite on WWOX and FHIT proteins and mRNA expression. In conclusion, arsenite decreased expressions of ATR, WWOX and FHIT via ERK1/2 activation in SV-HUC-1 cells. These findings confirm that dysregulations of these markers may contribute to arsenite-induced carcinogenesis.<br /> (Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.)
- Subjects :
- Acid Anhydride Hydrolases biosynthesis
Aged
Animals
Ataxia Telangiectasia Mutated Proteins
Cell Cycle Proteins biosynthesis
Cell Cycle Proteins genetics
Cell Line
Down-Regulation drug effects
Epithelial Cells enzymology
Epithelial Cells pathology
Epithelial Cells physiology
Female
Humans
Immunohistochemistry
Male
Neoplasm Proteins biosynthesis
Oxidoreductases biosynthesis
Protein Serine-Threonine Kinases biosynthesis
Protein Serine-Threonine Kinases genetics
Rats
Rats, Inbred F344
Taiwan epidemiology
Tumor Suppressor Proteins biosynthesis
Urologic Neoplasms enzymology
Urologic Neoplasms epidemiology
WW Domain-Containing Oxidoreductase
Acid Anhydride Hydrolases genetics
Arsenites poisoning
Arsenites toxicity
Epithelial Cells drug effects
Neoplasm Proteins genetics
Oxidoreductases genetics
Tumor Suppressor Proteins genetics
Urologic Neoplasms chemically induced
Urologic Neoplasms genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3169
- Volume :
- 220
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Toxicology letters
- Publication Type :
- Academic Journal
- Accession number :
- 23618899
- Full Text :
- https://doi.org/10.1016/j.toxlet.2013.04.007