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Characterization of Salmonella enterica subsp. enterica serovar 4,[5],12:i:- clones isolated from human and other sources in Switzerland between 2007 and 2011.
- Source :
-
Foodborne pathogens and disease [Foodborne Pathog Dis] 2013 Jun; Vol. 10 (6), pp. 549-54. Date of Electronic Publication: 2013 Apr 24. - Publication Year :
- 2013
-
Abstract
- Salmonella enterica subsp. enterica serovar 4,[5],12:i:- is a monophasic variant of Salmonella Typhimurium. In this study, a total of 651 human and 107 food and environmental isolates of serovar 4,[5],12:i:- recovered from 2007 through 2011 in Switzerland were characterized by antibiotic resistance profiles and pulsed-field gel electrophoresis (PFGE). In addition, a selection of isolates belonging to the most frequent PFGE patterns was further subjected to multilocus variable-number tandem-repeat analysis (MLVA) and phage typing. Over the years 2007-2011, the reports of salmonellosis caused by Salmonella enterica serovar 4,[5],12:i:- significantly increased. A high prevalence of multidrug-resistant isolates, mainly showing an ampicillin-streptomycin-sulfonamide-tetracycline resistance pattern (ASSuT), was observed. In addition, four extended spectrum beta lactamase (ESBL) (CTX-M-55)-producing isolates were found. XbaI PFGE analysis of all isolates revealed over 150 different pulsotypes, and generally showed a considerable diversity within the monophasic isolates. Nevertheless, among these we identified seven dominant profiles, which encompassed 66% of all isolates tested. The PFGE type STYMXB.0131 dominated among human as well as food isolates. Multilocus variable-number tandem-repeat analysis profile 3-12-10-0-0211, which, in many cases, coincided with PFGE type STYMXB.0131 and phage type DT193 were the most prevalent types found for the isolates further characterized by these typing methods. Our data provide strong evidence for a spread of two specific Salmonella serovar 4,[5],12:i:- clones (PFGE pattern STYMXB.0131, resistance type ASSuT) and (PFGE pattern STYMXB.0131, resistance type SSuT). In contrast to the human isolates, the pork/poultry isolates expressed predominantly the SSuT resistance type.
- Subjects :
- Animals
Bacterial Proteins genetics
Bacterial Proteins metabolism
Bacteriophage Typing
Bacteriophages classification
Bacteriophages growth & development
Bacteriophages isolation & purification
Chickens microbiology
Environmental Monitoring
Feces microbiology
Humans
Microbial Sensitivity Tests
Multilocus Sequence Typing
Salmonella enterica classification
Salmonella enterica isolation & purification
Salmonella enterica virology
Salmonella typhimurium classification
Salmonella typhimurium isolation & purification
Salmonella typhimurium virology
Site-Specific DNA-Methyltransferase (Adenine-Specific) genetics
Site-Specific DNA-Methyltransferase (Adenine-Specific) metabolism
Sus scrofa microbiology
Switzerland
Anti-Bacterial Agents pharmacology
Drug Resistance, Multiple, Bacterial
Meat microbiology
Salmonella Infections microbiology
Salmonella enterica drug effects
Salmonella typhimurium drug effects
Water Microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1556-7125
- Volume :
- 10
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Foodborne pathogens and disease
- Publication Type :
- Academic Journal
- Accession number :
- 23614800
- Full Text :
- https://doi.org/10.1089/fpd.2012.1407