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Hepatitis B virus induces expression of cholesterol metabolism-related genes via TLR2 in HepG2 cells.
- Source :
-
World journal of gastroenterology [World J Gastroenterol] 2013; Vol. 19 (14), pp. 2262-9. - Publication Year :
- 2013
-
Abstract
- Aim: To investigate whether hepatitis B virus (HBV) exacerbates hepatic cholesterol accumulation, and explore the underlying mechanisms.<br />Methods: HepG2 cells were infected with adenovirus (Ad) containing 1.3-fold overlength HBV genome. Real-time polymerase chain reaction and Western blotting were used to measure mRNA and protein expression of target genes. Cholesterol accumulation was measured by fluorescence microscopy. Cell toxicity due to Ad-HBV treatment was determined by the mitochondrial tetrazolium assay. The protein levels of toll-like receptors (TLRs) were determined by Western blotting.<br />Results: Ad-HBV increased hepatic cholesterol accumulation and enhanced the mRNA and protein levels of low-density lipoprotein receptor (LDLR) and 3-hydroxy-3-methylglutharyl-coenzyme A reductase (HMGCoAr) mRNA and protein expression in HepG2 cells. In addition, these inductive effects were partly offset by suppressing TLR2 expression levels by small interfering RNA in HepG2 cells.<br />Conclusion: Ad-HBV increases LDLR and HMGCoAr expression, resulting in exacerbated cholesterol accumulation in HepG2 cells, which was mediated via the TLR2 pathway.
- Subjects :
- Adenoviridae genetics
Genetic Vectors
Hep G2 Cells
Hepatitis B virus genetics
Hepatitis B virus immunology
Hepatocytes immunology
Hepatocytes virology
Humans
Hydroxymethylglutaryl CoA Reductases genetics
Hydroxymethylglutaryl CoA Reductases metabolism
RNA Interference
RNA, Messenger metabolism
Receptors, LDL genetics
Receptors, LDL metabolism
Toll-Like Receptor 2 genetics
Transduction, Genetic
Up-Regulation
Cholesterol metabolism
Hepatitis B virus metabolism
Hepatocytes metabolism
Toll-Like Receptor 2 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2219-2840
- Volume :
- 19
- Issue :
- 14
- Database :
- MEDLINE
- Journal :
- World journal of gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 23599654
- Full Text :
- https://doi.org/10.3748/wjg.v19.i14.2262