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QCM immunoassay for recombinant cysteine peptidase: a potential protein biomarker for diagnosis of citrus canker.
- Source :
-
Talanta [Talanta] 2013 Jan 30; Vol. 104, pp. 193-7. Date of Electronic Publication: 2012 Nov 13. - Publication Year :
- 2013
-
Abstract
- Citrus canker is one of the most important agricultural citrus diseases worldwide. It is caused by Xanthomonas citri subsp. citri (Xcc) bacterium that infects leaves and the fruits produce a cysteine peptidase (CPXaC), which makes it a potential target for the development of effective and rapid detection methods for citrus canker. We report here the studies on the development of piezoelectric immunoassay for CPXaC using a polyclonal antibody against CPXaC (anti-CPXaC). Three different strategies for covalent immobilization of anti-CPXaC on gold surfaces were evaluated by monitoring the frequency (Δf) and energy dissipation (ΔD) variation in real time when 64.5×10(-8) mol L(-1) CPXaC was added. Anti-CPXaC immobilized with 11-mercaptoundecanoic acid (MUA) showed the best relation between the frequency and dissipation factor variation, and strong values for the kinetic and equilibrium binding constant were obtained. The immunosensor showed a detection limit of 13.0 nmol L(-1) with excellent specificity, showing no response for different proteins that include another cysteine peptidase that is used as a target to detect Xylella fastidiosa bacterium, responsible for another important citrus disease. These results provide good perspectives for the use of CPXaC as a new biomarker for citrus canker.<br /> (Copyright © 2012 Elsevier B.V. All rights reserved.)
- Subjects :
- Antibodies chemistry
Antibodies immunology
Biomarkers
Citrus
Cysteine Proteases immunology
Gold chemistry
Immobilized Proteins chemistry
Immobilized Proteins immunology
Immunoassay
Recombinant Proteins analysis
Recombinant Proteins immunology
Cysteine Proteases analysis
Plant Diseases
Xanthomonas enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3573
- Volume :
- 104
- Database :
- MEDLINE
- Journal :
- Talanta
- Publication Type :
- Academic Journal
- Accession number :
- 23597909
- Full Text :
- https://doi.org/10.1016/j.talanta.2012.11.003