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Pseudovirus-based neuraminidase inhibition assays reveal potential H5N1 drug-resistant mutations.
- Source :
-
Protein & cell [Protein Cell] 2013 May; Vol. 4 (5), pp. 356-63. Date of Electronic Publication: 2013 Apr 15. - Publication Year :
- 2013
-
Abstract
- The use of antiviral drugs such as influenza neuraminidase (NA) inhibitors is a critical strategy to prevent and control flu pandemic, but this strategy faces the challenge of emerging drug-resistant strains. For a highly pathogenic avian influenza (HPAI) H5N1 virus, biosafety restrictions have significantly limited the efforts to monitor its drug responses and mechanisms involved. In this study, a rapid and biosafe assay based on NA pseudovirus was developed to study the resistance of HPAI H5N1 virus to NA inhibitor drugs. The H5N1 NA pseudovirus was comprehensively tested using oseltamivir-sensitive strains and their resistant mutants. Results were consistent with those in previous studies, in which live H5N1 viruses were used. Several oseltamivir-resistant mutations reported in human H1N1 were also identified to cause decreased oseltamivir sensitivity in H5N1 NA by using the H5N1 NA pseudovirus. Thus, H5N1 NA pseudoviruses could be used to monitor HPAI H5N1 drug resistance rapidly and safely.
- Subjects :
- Animals
Birds
Enzyme Inhibitors therapeutic use
HEK293 Cells
Humans
Influenza A Virus, H1N1 Subtype drug effects
Influenza A Virus, H1N1 Subtype genetics
Influenza A Virus, H1N1 Subtype pathogenicity
Influenza A Virus, H5N1 Subtype drug effects
Influenza A Virus, H5N1 Subtype pathogenicity
Influenza in Birds drug therapy
Influenza in Birds genetics
Influenza in Birds virology
Influenza, Human drug therapy
Influenza, Human virology
Mutagenesis, Site-Directed
Neuraminidase genetics
Oseltamivir administration & dosage
Drug Resistance, Viral genetics
Influenza A Virus, H5N1 Subtype genetics
Influenza, Human genetics
Neuraminidase antagonists & inhibitors
Subjects
Details
- Language :
- English
- ISSN :
- 1674-8018
- Volume :
- 4
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Protein & cell
- Publication Type :
- Academic Journal
- Accession number :
- 23589018
- Full Text :
- https://doi.org/10.1007/s13238-013-2125-y