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Isolation, purification, culture and characterisation of myoepithelial cells from normal and neoplastic canine mammary glands using a magnetic-activated cell sorting separation system.
- Source :
-
Veterinary journal (London, England : 1997) [Vet J] 2013 Aug; Vol. 197 (2), pp. 474-82. Date of Electronic Publication: 2013 Apr 11. - Publication Year :
- 2013
-
Abstract
- Mammary gland tumours, the most common malignant neoplasm in bitches, often display myoepithelial (ME) cell proliferation. The aim of this study was to isolate, purify, culture and characterise ME cells from normal and neoplastic canine mammary glands. Monodispersed cells from three normal canine mammary glands and five canine mammary tumours were incubated with an anti-Thy1 antibody and isolated by magnetic-activated cell sorting (MACS). Cells isolated from two normal glands (cell lines CmME-N1 and CmME-N2) and four tumours (cell lines CmME-K1 from a complex carcinoma, CmME-K2 from a simple tubulopapillary carcinoma, and CmME-K3 and CmME-K4 from two carcinomas within benign tumours) were cultured in supplemented DMEM/F12 media for 40days. Cell purity was >90%. Tumour-derived ME cell lines exhibited heterogeneous morphology, growth patterns and immunocytochemical expression of cytokeratins, whereas cell lines from normal glands retained their morphology and levels of cytokeratin expression during culture. Cell lines from normal glands and carcinomas within benign tumours grew more slowly than those from simple and complex carcinomas. This methodology has the potential to be used for in vitro analysis of the role of ME cells in the growth and progression of canine mammary tumours.<br /> (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Dogs
Epithelial Cells cytology
Epithelial Cells physiology
Female
Gene Expression Regulation physiology
Immunohistochemistry
Myoepithelioma pathology
Thy-1 Antigens genetics
Thy-1 Antigens metabolism
Cell Culture Techniques veterinary
Dog Diseases pathology
Epithelial Cells classification
Mammary Glands, Animal cytology
Mammary Neoplasms, Animal pathology
Myoepithelioma veterinary
Subjects
Details
- Language :
- English
- ISSN :
- 1532-2971
- Volume :
- 197
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Veterinary journal (London, England : 1997)
- Publication Type :
- Academic Journal
- Accession number :
- 23583698
- Full Text :
- https://doi.org/10.1016/j.tvjl.2013.03.005