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The inhibin-βC subunit is down-regulated, while inhibin-βE is up-regulated by interferon-β1a in Ishikawa carcinoma cell line.
- Source :
-
Archives of gynecology and obstetrics [Arch Gynecol Obstet] 2013 Oct; Vol. 288 (4), pp. 883-8. Date of Electronic Publication: 2013 Apr 12. - Publication Year :
- 2013
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Abstract
- Introduction: Inhibins are important regulators of the female reproductive system. Recently, two new inhibin-subunits βC and βE have been described, although, their function is still quite unclear. Interestingly, there is an association between interferon and TGF-β expression. Therefore, the aim of this study was to determine expression changes of inhibin-βC and -βE subunits in endometrial Ishikawa carcinoma cell line after stimulation with interferon-β1a.<br />Materials and Methods: The Ishikawa cell line was cultured until confluence was observed (after 2 days). After adding interferon-β1a (1,000 IE/ml), Ishikawa cells were analyzed for inhibin-βC and -βE subunits by RT-PCR. The fibroblast cell line BJ6 served as negative control. Experiments were performed in triplicates.<br />Results: The endometrial adenocarcinoma cell line Ishikawa synthesized the inhibin- βC and -βE subunits. The fibroblast cells BJ6 did not demonstrate an inhibin -βC and -βE mRNA expression, while inhibin-βC subunit is down-regulated and inhibin-βE is up-regulated in Ishikawa carcinoma cell line after stimulation with interferon-β1a in Ishikawa.<br />Discussion: We demonstrated for the first time a functional relationship between interferon and the novel inhibin-βC and -βE subunits. It might be possible that interferon exerts a possible apoptotic function through the βE-subunit, while, by down-regulating the βC isoform, cell proliferation is inhibited. However, the precise function of the novel βC- and βE-subunits are still not known in human endometrial tissue and a possible association with interferon is still unclear and warrants further research.
- Subjects :
- Adenocarcinoma metabolism
Biomarkers, Tumor metabolism
Cell Line, Tumor
Down-Regulation
Endometrial Neoplasms metabolism
Female
Humans
Inhibin-beta Subunits metabolism
Interferon beta-1a
Interferon-beta metabolism
Reverse Transcriptase Polymerase Chain Reaction
Up-Regulation
Adenocarcinoma genetics
Biomarkers, Tumor genetics
Endometrial Neoplasms genetics
Gene Expression Regulation, Neoplastic
Inhibin-beta Subunits genetics
Interferon-beta genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1432-0711
- Volume :
- 288
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Archives of gynecology and obstetrics
- Publication Type :
- Academic Journal
- Accession number :
- 23580013
- Full Text :
- https://doi.org/10.1007/s00404-013-2848-2