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Requirement for core 2 O-glycans for optimal resistance to helminth infection.
- Source :
-
PloS one [PLoS One] 2013; Vol. 8 (3), pp. e60124. Date of Electronic Publication: 2013 Mar 29. - Publication Year :
- 2013
-
Abstract
- The migration of lymphocytes to the small intestine is controlled by expression of the integrin α4β7 and the chemokine receptor CCR9. However, the molecules that specifically regulate migration to the large intestine remain unclear. Immunity to infection with the large intestinal helminth parasite Trichuris muris is dependent upon CD4(+) T cells that migrate to the large intestine. We examine the role of specific chemokine receptors, adhesion molecules and glycosyltransferases in the development of protective immunity to Trichuris. Mice deficient in expression of the chemokine receptors CCR2 or CCR6 were resistant to infection with Trichuris. Similarly, loss of CD34, CD43, CD44 or PSGL-1 had no effect on resistance to infection. In contrast, simultaneous deletion of the Core2 β1,6-N-acetylglucosaminyltransferase (C2GnT) enzymes C2GnT1 and C2Gnt2 resulted in delayed expulsion of worms. These results suggest that C2GnT-dependent modifications may play a role in migration of protective immune cells to the large intestine.
- Subjects :
- Animals
Antigens, CD34 genetics
Antigens, CD34 metabolism
CD4-Positive T-Lymphocytes metabolism
Hyaluronan Receptors genetics
Hyaluronan Receptors metabolism
Leukosialin genetics
Leukosialin metabolism
Membrane Glycoproteins genetics
Membrane Glycoproteins metabolism
Mice
Mice, Inbred C57BL
Mice, Knockout
N-Acetylglucosaminyltransferases genetics
N-Acetylglucosaminyltransferases metabolism
Real-Time Polymerase Chain Reaction
Receptors, CCR2 genetics
Receptors, CCR2 metabolism
Receptors, CCR6 genetics
Receptors, CCR6 metabolism
Trichuriasis genetics
Intestine, Large metabolism
Intestine, Large parasitology
Polysaccharides metabolism
Trichuriasis metabolism
Trichuris pathogenicity
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 8
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 23555902
- Full Text :
- https://doi.org/10.1371/journal.pone.0060124