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Ferumoxytol: a new, clinically applicable label for stem-cell tracking in arthritic joints with MRI.
- Source :
-
Nanomedicine (London, England) [Nanomedicine (Lond)] 2013 Dec; Vol. 8 (12), pp. 1969-83. Date of Electronic Publication: 2013 Mar 27. - Publication Year :
- 2013
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Abstract
- Aim: To develop a clinically applicable MRI technique for tracking stem cells in matrix-associated stem-cell implants, using the US FDA-approved iron supplement ferumoxytol.<br />Materials & Methods: Ferumoxytol-labeling of adipose-derived stem cells (ADSCs) was optimized in vitro. A total of 11 rats with osteochondral defects of both femurs were implanted with ferumoxytol- or ferumoxides-labeled or unlabeled ADSCs, and underwent MRI up to 4 weeks post matrix-associated stem-cell implant. The signal-to-noise ratio of different matrix-associated stem-cell implant was compared with t-tests and correlated with histopathology.<br />Results: An incubation concentration of 500 µg iron/ml ferumoxytol and 10 µg/ml protamine sulfate led to significant cellular iron uptake, T2 signal effects and unimpaired ADSC viability. In vivo, ferumoxytol- and ferumoxides-labeled ADSCs demonstrated significantly lower signal-to-noise ratio values compared with unlabeled controls (p < 0.01). Histopathology confirmed engraftment of labeled ADSCs, with slow dilution of the iron label over time.<br />Conclusion: Ferumoxytol can be used for in vivo tracking of stem cells with MRI.
- Subjects :
- Animals
Arthritis pathology
Arthritis surgery
Cells, Cultured
Female
Femur pathology
Femur surgery
Joints pathology
Joints surgery
Parenteral Nutrition Solutions analysis
Rats
Rats, Nude
Cell Tracking methods
Contrast Media analysis
Ferrosoferric Oxide analysis
Magnetic Resonance Imaging methods
Stem Cell Transplantation
Stem Cells cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1748-6963
- Volume :
- 8
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Nanomedicine (London, England)
- Publication Type :
- Academic Journal
- Accession number :
- 23534832
- Full Text :
- https://doi.org/10.2217/nnm.12.198