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Distribution and infection-related functions of bacillithiol in Staphylococcus aureus.
- Source :
-
International journal of medical microbiology : IJMM [Int J Med Microbiol] 2013 Apr; Vol. 303 (3), pp. 114-23. Date of Electronic Publication: 2013 Mar 06. - Publication Year :
- 2013
-
Abstract
- Bacillithiol (Cys-GlcN-malate, BSH) serves as a major low molecular weight thiol in low GC Gram-positive bacteria including Bacillus species and a variety of Staphylococcus aureus strains. These bacteria do not produce glutathione (GSH). In this study, HPLC analyses were used to determine BSH levels in different S. aureus strains. Furthermore, the role of BSH in the resistance against oxidants and antibiotics and its function in virulence was investigated. We and others (Newton, G.L., Fahey, R.C., Rawat, M., 2012. Microbiology 158, 1117-1126) found that BSH is not produced by members of the S. aureus NCTC8325 lineage, such as strains 8325-4 and SH1000. Using bioinformatics we show that the BSH-biosynthetic gene bshC is disrupted by an 8-bp duplication in S. aureus NCTC8325. The functional bshC-gene from BSH-producing S. aureus Newman (NWMN&#95;1087) was expressed in S. aureus 8325-4 to reconstitute BSH-synthesis. Comparison of the BSH-producing and BSH-minus strains revealed higher resistance of the BSH-producing strain against the antibiotic fosfomycin and the oxidant hypochlorite but not against hydrogen peroxide or diamide. In addition, a higher bacterial load of the BSH-producing strain was detected in human upper-airway epithelial cells and murine macrophages. This indicates a potential role of BSH in protection of S. aureus during infection.<br /> (Copyright © 2013 Elsevier GmbH. All rights reserved.)
- Subjects :
- Animals
Anti-Bacterial Agents pharmacology
Antioxidants
Bacterial Load
Cell Line
Chromatography, High Pressure Liquid
Cysteine biosynthesis
Cysteine genetics
Diamide pharmacology
Drug Resistance, Bacterial
Epithelial Cells microbiology
Fosfomycin pharmacology
Gene Expression
Glucosamine biosynthesis
Glucosamine genetics
Humans
Hydrogen Peroxide pharmacology
Hypochlorous Acid pharmacology
Macrophages microbiology
Mice
Oxidants pharmacology
Staphylococcus aureus chemistry
Staphylococcus aureus genetics
Virulence Factors genetics
Cysteine analogs & derivatives
Glucosamine analogs & derivatives
Staphylococcus aureus metabolism
Staphylococcus aureus pathogenicity
Virulence Factors biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1618-0607
- Volume :
- 303
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- International journal of medical microbiology : IJMM
- Publication Type :
- Academic Journal
- Accession number :
- 23517692
- Full Text :
- https://doi.org/10.1016/j.ijmm.2013.01.003