Back to Search
Start Over
Indirect fluorescent antibody test and surface antigen ELISAs for antemortem diagnosis of equine protozoal myeloencephalitis.
- Source :
-
Journal of veterinary internal medicine [J Vet Intern Med] 2013 May-Jun; Vol. 27 (3), pp. 596-9. Date of Electronic Publication: 2013 Mar 20. - Publication Year :
- 2013
-
Abstract
- Background: Recent research suggests that serum : CSF titer ratios could provide the most accurate antemortem diagnosis of equine protozoal myeloencephalitis (EPM) caused by Sarcocystis neurona.<br />Objectives: The purpose of this study was to assess the utility of two commercially available tests, the indirect fluorescent antibody test (IFAT) and the surface antigen 2, 4/3 ELISA (SAG2, 4/3 ELISA), using archived paired serum and CSF samples.<br />Animals: Samples were obtained from 4 types of clinical patients. Confirmed positive cases (n = 9 horses; 11 sample sets) had neurologic deficits and postmortem lesions consistent with EPM. Confirmed negative cases (n = 28) had variable clinical signs and postmortem lesions consistent with another disease. Suspected positive cases (n = 6) had neurologic deficits consistent with EPM, marked improvement after treatment, and exclusion of other diseases. Suspected negative cases (n = 14) had variable signs with a strong presumptive diagnosis of another disease.<br />Methods: For each test, descriptive statistics were calculated using serum results alone, CSF results alone, and a serum : CSF titer ratio.<br />Results: Overall accuracy was highest for SAG2, 4/3 ELISA titer ratio at 0.97 (95% CI 0.88-0.99) with sensitivity = 0.88 (95% CI 0.66-0.97) and specificity = 1 (95% CI 0.92-1). IFAT CSF and titer ratio results also showed high accuracy at 0.88 (95% CI 0.77-0.94), but lower sensitivity = 0.65 (95% CI 0.41-0.83).<br />Conclusions and Clinical Importance: Using serum results alone was least accurate for both test types. The more accurate methods, such as the SAG2, 4/3 ELISA serum : CSF titer ratio, should be utilized.<br /> (Copyright © 2013 by the American College of Veterinary Internal Medicine.)
- Subjects :
- Animals
Antigens, Surface blood
Encephalomyelitis parasitology
Horse Diseases diagnosis
Horses
Sarcocystis metabolism
Sarcocystosis diagnosis
Antigens, Protozoan isolation & purification
Encephalomyelitis veterinary
Enzyme-Linked Immunosorbent Assay veterinary
Fluorescent Antibody Technique, Indirect veterinary
Horse Diseases parasitology
Sarcocystosis veterinary
Subjects
Details
- Language :
- English
- ISSN :
- 1939-1676
- Volume :
- 27
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of veterinary internal medicine
- Publication Type :
- Academic Journal
- Accession number :
- 23517480
- Full Text :
- https://doi.org/10.1111/jvim.12061