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Characterization and genome sequencing of a novel bacteriophage infecting Streptococcus agalactiae with high similarity to a phage from Streptococcus pyogenes.

Authors :
Bai Q
Zhang W
Yang Y
Tang F
Nguyen X
Liu G
Lu C
Source :
Archives of virology [Arch Virol] 2013 Aug; Vol. 158 (8), pp. 1733-41. Date of Electronic Publication: 2013 Mar 21.
Publication Year :
2013

Abstract

A novel bacteriophage, JX01, specifically infecting bovine Streptococcus agalactiae was isolated from milk of mastitis-affected cattle. The phage morphology showed that JX01 belongs to the family Siphoviridae, and this phage demonstrated a broad host range. Microbiological characterization demonstrated that nearly 90 % of JX01 phage particles were adsorbed after 2.5 min of incubation, that the burst size was 20 virions released per infected host cell, and that there was a latent period of 30 min. JX01 was thermal sensitive and showed acid and alkaline resistance (pH 3-11). The genome of JX01 was found to consist of a linear, double-stranded 43,028-bp DNA molecule with a GC content of 36.81 % and 70 putative open reading frames (ORFs) plus one tRNA. Comparative genome analysis revealed high similarity between JX01 and the prophage 315.2 of Streptococcus pyogenes.

Details

Language :
English
ISSN :
1432-8798
Volume :
158
Issue :
8
Database :
MEDLINE
Journal :
Archives of virology
Publication Type :
Academic Journal
Accession number :
23515875
Full Text :
https://doi.org/10.1007/s00705-013-1667-x