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Characterization and genome sequencing of a novel bacteriophage infecting Streptococcus agalactiae with high similarity to a phage from Streptococcus pyogenes.
- Source :
-
Archives of virology [Arch Virol] 2013 Aug; Vol. 158 (8), pp. 1733-41. Date of Electronic Publication: 2013 Mar 21. - Publication Year :
- 2013
-
Abstract
- A novel bacteriophage, JX01, specifically infecting bovine Streptococcus agalactiae was isolated from milk of mastitis-affected cattle. The phage morphology showed that JX01 belongs to the family Siphoviridae, and this phage demonstrated a broad host range. Microbiological characterization demonstrated that nearly 90 % of JX01 phage particles were adsorbed after 2.5 min of incubation, that the burst size was 20 virions released per infected host cell, and that there was a latent period of 30 min. JX01 was thermal sensitive and showed acid and alkaline resistance (pH 3-11). The genome of JX01 was found to consist of a linear, double-stranded 43,028-bp DNA molecule with a GC content of 36.81 % and 70 putative open reading frames (ORFs) plus one tRNA. Comparative genome analysis revealed high similarity between JX01 and the prophage 315.2 of Streptococcus pyogenes.
- Subjects :
- Animals
Base Composition
Cattle
Cluster Analysis
Molecular Sequence Data
Open Reading Frames
Phylogeny
Sequence Analysis, DNA
Sequence Homology
Siphoviridae genetics
Siphoviridae isolation & purification
Siphoviridae physiology
Siphoviridae ultrastructure
Streptococcus Phages physiology
Streptococcus Phages ultrastructure
Streptococcus agalactiae isolation & purification
Virus Attachment
DNA, Viral chemistry
DNA, Viral genetics
Genome, Viral
Streptococcus Phages genetics
Streptococcus Phages isolation & purification
Streptococcus agalactiae virology
Subjects
Details
- Language :
- English
- ISSN :
- 1432-8798
- Volume :
- 158
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Archives of virology
- Publication Type :
- Academic Journal
- Accession number :
- 23515875
- Full Text :
- https://doi.org/10.1007/s00705-013-1667-x