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Detection of orexin A neuropeptide in biological fluids using a zinc oxide field effect transistor.
- Source :
-
ACS chemical neuroscience [ACS Chem Neurosci] 2013 Mar 20; Vol. 4 (3), pp. 444-53. Date of Electronic Publication: 2013 Jan 22. - Publication Year :
- 2013
-
Abstract
- Biomarkers which are indicative of acute physiological and emotional states are studied in a number of different areas in cognitive neuroscience. Currently, many cognitive studies are conducted based on programmed tasks followed by timed biofluid sampling, central laboratory processing, and followed by data analysis. In this work, we present a sensor platform capable of rapid biomarker detection specific for detecting neuropeptide orexin A, found in blood and saliva and known as an indicator of fatigue and cognitive performance. A peptide recognition element that selectively binds to orexin A was designed, characterized, and functionalized onto a zinc oxide field effect transistor to enable rapid detection. The detection limit using the sensor platform was sub-picomolar in water, and picomolar to nanomolar levels in saliva and serum. The transistor and recognition element sensor platform can be easily expanded, allowing for multiple biomarkers to be detected simultaneously, lending itself to complex biomarker analysis applicable to rapid feedback for neuroscience research and physiological monitoring.
- Subjects :
- Amino Acid Sequence
Animals
Biomarkers blood
Biomarkers metabolism
Biosensing Techniques instrumentation
Humans
Intracellular Signaling Peptides and Proteins metabolism
Mice
Molecular Sequence Data
Neuropeptides metabolism
Orexins
Rats
Saliva metabolism
Serum metabolism
Biosensing Techniques methods
Intracellular Signaling Peptides and Proteins chemistry
Neuropeptides chemistry
Saliva chemistry
Serum chemistry
Transistors, Electronic
Zinc Oxide metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1948-7193
- Volume :
- 4
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- ACS chemical neuroscience
- Publication Type :
- Academic Journal
- Accession number :
- 23509980
- Full Text :
- https://doi.org/10.1021/cn300159e