Amino acid substitutions in GyrA and ParC are associated with fluoroquinolone resistance in Mycoplasma bovis isolates from Japanese dairy calves.

Authors :: Sato T
Okubo T
Usui M
Higuchi H
Tamura Y
Source :: The Journal of veterinary medical science [J Vet Med Sci] 2013; Vol. 75 (8), pp. 1063-5. Date of Electronic Publication: 2013 Mar 15.
Publication Year :: 2013
Abstract: We investigated the contribution of quinolone resistance-determining region (QRDR) mutations to fluoroquinolone (enrofloxacin, orbifloxacin and danofloxacin) susceptibility in 58 Mycoplasma bovis isolates from dairy cattle in Japan. Fluoroquinolone non-resistant isolates (fluoroquinolone-MICs≤2 μg/ml) possessed no QRDR mutations (19 isolates) or Ser83Leu in GyrA (32 isolates). Fluoroquinolone-resistant isolates (fluoroquinolone-MICs≥4 μg/ml) possessed Ser83Leu in GyrA and Ser81Pro in ParC (3 isolates) or Ser83Phe in GyrA and Ser80Ile in ParC (4 isolates). Laboratory-derived fluoroquinolone-resistant mutants selected from 2 isolates (possessing Ser83Leu in GyrA) had an amino acid substitution in ParC at the same position (Ser80Ile or Ser81Tyr) as fluoroquinolone-resistant isolates, suggesting a concurrent amino acid substitution in ParC (Ser80 or Ser81) is important in fluoroquinolone resistance in M. bovis isolates.

Subjects :: Amino Acid Substitution
Animals
Cattle
Cattle Diseases microbiology
Japan
Microbial Sensitivity Tests
Mycoplasma Infections drug therapy
Mycoplasma bovis drug effects
Cattle Diseases drug therapy
DNA Gyrase genetics
DNA Topoisomerase IV genetics
Drug Resistance, Fungal genetics
Fluoroquinolones pharmacology
Mycoplasma Infections veterinary
Mycoplasma bovis genetics

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