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Modulation of angiogenic factor VEGF by DNA-hsp65 vaccination in a murine CNS tuberculosis model.
- Source :
-
Tuberculosis (Edinburgh, Scotland) [Tuberculosis (Edinb)] 2013 May; Vol. 93 (3), pp. 373-80. Date of Electronic Publication: 2013 Mar 13. - Publication Year :
- 2013
-
Abstract
- Tuberculosis (TB) is a serious public health problem. Development of experimental models and vaccines are essential to elucidate physiopathological mechanisms and to control the disease. Vascular endothelial growth factor (VEGF) is a potent activator of vascular permeability and angiogenesis. VEGF seems to participate in breakdown of the blood brain-barrier (BBB) in tuberculous meningitis (TBM), contributing to worsening of disease. Therefore, the objective here was to extent the characterization of our previously described murine model of central nervous system TB (CNS-TB) by describing the VEGF participation in the CNS disease, and suggesting a vaccination plan in mice. Plasmid encoding DNA protein antigen DNA-hsp65 has been described as a protector against TB infection and was used here to test its effectiveness in the prevention of VEGF production and TB disease. Vaccinated mice and its controls were injected with Mycobacterium bovis bacillus Calmette-Guerin (BCG) in cerebellum. Four weeks after BCG injection, mice were perfused and brains were paraffin-embedded for VEGF expression analysis. We observed VEGF immunohistochemical expression in TBM and granulomas in non-vaccinated mice. The DNA-hsp65 treatment blocked the expression of VEGF in mice TBM. Therefore, our murine model indicated the VEGF participation in the physiopathology of CNS-TB and the potential prevention of the DNA-hsp65 in the disease progression.<br /> (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Bacterial Proteins genetics
Cerebellar Diseases metabolism
Cerebellar Diseases prevention & control
Cerebellum metabolism
Chaperonin 60 genetics
Disease Models, Animal
Immunization Schedule
Male
Mice
Mycobacterium bovis
Tuberculoma, Intracranial metabolism
Tuberculoma, Intracranial prevention & control
Tuberculosis, Central Nervous System prevention & control
Tuberculosis, Meningeal metabolism
Tuberculosis, Meningeal prevention & control
Bacterial Proteins immunology
Chaperonin 60 immunology
Tuberculosis Vaccines immunology
Tuberculosis, Central Nervous System metabolism
Vaccines, DNA immunology
Vascular Endothelial Growth Factor A biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1873-281X
- Volume :
- 93
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Tuberculosis (Edinburgh, Scotland)
- Publication Type :
- Academic Journal
- Accession number :
- 23491717
- Full Text :
- https://doi.org/10.1016/j.tube.2013.02.002