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Identification of specific regions in hepatitis C virus core, NS2 and NS5A that genetically interact with p7 and co-ordinate infectious virus production.
- Source :
-
Journal of viral hepatitis [J Viral Hepat] 2013 Apr; Vol. 20 (4), pp. e66-71. Date of Electronic Publication: 2012 Sep 30. - Publication Year :
- 2013
-
Abstract
- The p7 protein of hepatitis C virus (HCV) is a small, integral membrane protein that plays a critical role in virus replication. Recently, we reported two intergenotypic JFH1 chimeric viruses encoding the partial or full-length p7 protein of the HCV-A strain of genotype 1b (GT1b; Virology; 2007; 360:134). In this study, we determined the consensus sequences of the entire polyprotein coding regions of the wild-type JFH1 and the revertant chimeric viruses and identified predominant amino acid substitutions in core (K74M), NS2 (T23N, H99P) and NS5A (D251G). Forward genetic analysis demonstrated that all single mutations restored the infectivity of the defective chimeric genomes suggesting that the infectious virus production involves the association of p7 with specific regions in core, NS2 and NS5A. In addition, it was demonstrated that the NS2 T23N facilitated the generation of infectious intergenotypic chimeric virus encoding p7 from GT6 of HCV.<br /> (© 2012 Blackwell Publishing Ltd.)
- Subjects :
- DNA Mutational Analysis
Hepacivirus genetics
Protein Interaction Mapping
Viral Core Proteins genetics
Viral Nonstructural Proteins genetics
Viral Proteins genetics
Hepacivirus physiology
Protein Interaction Domains and Motifs
Viral Core Proteins metabolism
Viral Nonstructural Proteins metabolism
Viral Proteins metabolism
Virus Replication
Subjects
Details
- Language :
- English
- ISSN :
- 1365-2893
- Volume :
- 20
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of viral hepatitis
- Publication Type :
- Academic Journal
- Accession number :
- 23490391
- Full Text :
- https://doi.org/10.1111/jvh.12004