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Highly efficient SiRNA and gene transfer into hepatocyte-like HepaRG cells and primary human hepatocytes: new means for drug metabolism and toxicity studies.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2013; Vol. 987, pp. 295-314. - Publication Year :
- 2013
-
Abstract
- The metabolically competent hepatocyte-like human HepaRG cells represent a suitable alternative in vitro cell model to human primary hepatocytes. Here, we describe the culture procedure required to expand progenitor HepaRG cells and to differentiate them into hepatocyte-like cells. Transient transfection of gene and siRNA into cultured cells, using nonviral strategies, is an invaluable technique to decipher gene functions. In this chapter, we detail transfection protocols for efficient transfer of plasmid DNA or siRNAs into proliferating progenitor or quiescent differentiated HepaRG cells as well as into primary hepatocytes.
- Subjects :
- Cell Culture Techniques
Cell Differentiation
Cell Proliferation
Electroporation
Hepatocytes cytology
Humans
Indicators and Reagents chemistry
Stem Cells cytology
Drug-Related Side Effects and Adverse Reactions
Hepatocytes metabolism
Pharmaceutical Preparations metabolism
RNA, Small Interfering genetics
Transfection methods
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 987
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 23475687
- Full Text :
- https://doi.org/10.1007/978-1-62703-321-3_25