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Bioluminescent assays for cytochrome P450 enzymes.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2013; Vol. 987, pp. 1-9. - Publication Year :
- 2013
-
Abstract
- The cytochrome P450 (CYP) family contains 57 enzymes in humans. The activity of CYPs against xenobiotics is a primary consideration in drug optimization efforts. Here we describe a series of bioluminescent assays that enable the rapid profiling of CYP activity against compound collections. The assays employ a coupled-enzyme format where firefly luciferase is used to measure CYP enzyme activity through metabolism of pro-luciferase substrates.
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 987
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 23475663
- Full Text :
- https://doi.org/10.1007/978-1-62703-321-3_1