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Isolation and screening of black fungi as degraders of volatile aromatic hydrocarbons.
- Source :
-
Mycopathologia [Mycopathologia] 2013 Jun; Vol. 175 (5-6), pp. 369-79. Date of Electronic Publication: 2013 Mar 09. - Publication Year :
- 2013
-
Abstract
- Black fungi reported as degraders of volatile aromatic compounds were isolated from hydrocarbon-polluted sites and indoor environments. Several of the species encountered are known opportunistic pathogens or are closely related to pathogenic species causing severe mycoses, among which are neurological infections in immunocompetent individuals. Given the scale of the problem of environmental pollution and the phylogenetic relation of aromate-degrading black fungi with pathogenic siblings, it is of great interest to select strains able to mineralize these substrates efficiently without any risk for public health. Fifty-six black strains were obtained from human-made environments rich in hydrocarbons (gasoline car tanks, washing machine soap dispensers) after enrichment with some phenolic intermediates of toluene and styrene fungal metabolism. Based on ITS sequencing identification, the majority of the obtained isolates were members of the genus Exophiala. Exophiala xenobiotica was found to be the dominant black yeast present in the car gasoline tanks. A higher biodiversity, with three Exophiala species, was found in soap dispensers of washing machines. Strains obtained were screened using a 2,6-dichlorophenol-indophenol (DCPIP) assay, optimized for black fungi, to assess their potential ability to degrade toluene. Seven out of twenty strains tested were able to use toluene as carbon source.
- Subjects :
- Ascomycota genetics
Ascomycota metabolism
Biotransformation
Carbon metabolism
Cluster Analysis
DNA, Fungal chemistry
DNA, Fungal genetics
DNA, Ribosomal Spacer chemistry
DNA, Ribosomal Spacer genetics
Humans
Molecular Sequence Data
Phylogeny
Sequence Analysis, DNA
Ascomycota classification
Ascomycota isolation & purification
Environmental Microbiology
Environmental Pollutants metabolism
Hydrocarbons, Aromatic metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1573-0832
- Volume :
- 175
- Issue :
- 5-6
- Database :
- MEDLINE
- Journal :
- Mycopathologia
- Publication Type :
- Academic Journal
- Accession number :
- 23475324
- Full Text :
- https://doi.org/10.1007/s11046-013-9635-2