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Urinary 1H-NMR metabolomics can distinguish pancreatitis patients from healthy controls.
- Source :
-
JOP : Journal of the pancreas [JOP] 2013 Mar 10; Vol. 14 (2), pp. 161-70. Date of Electronic Publication: 2013 Mar 10. - Publication Year :
- 2013
-
Abstract
- Context: The characterization of the urinary metabolome may yield biomarkers indicative of pancreatitis.<br />Objectives: We establish a non-invasive technique to compare urinary metabolic profiles in patients with acute and chronic pancreatitis to healthy controls.<br />Methods: Urine was obtained from healthy controls (HC, n=5), inpatients with mild acute pancreatitis (AP, n=5), and outpatients with chronic pancreatitis (CP, n=5). Proton nuclear magnetic resonance spectra were obtained for each sample. Metabolites were identified and quantified in each spectrum; resulting concentrations were normalized to account for differences in dilution among samples. Kruskal-Wallis test, post-hoc Mann-Whitney U tests, and principal component analysis were performed to identify metabolites that discriminate healthy controls, acute pancreatitis, and chronic pancreatitis.<br />Results: Sixty metabolites were identified and quantified; five were found to differ significantly (P&lt;0.05) among the three groups. Of these, citrate and adenosine remained significant after validation by random permutation. Principal component analysis demonstrated that healthy control urine samples can be differentiated from patients with chronic pancreatitis or acute pancreatitis; chronic pancreatitis patients could not be distinguished from acute pancreatitis patients.<br />Conclusions: This metabolomic investigation demonstrates that this non-invasive technique offers insight into the metabolic states of pancreatitis. Although the identified metabolites cannot conclusively be defined as biomarkers of disease, future studies will validate our findings in larger patient cohorts.
- Subjects :
- Adult
Aged
Case-Control Studies
Cohort Studies
Female
Health
Humans
Male
Metabolome physiology
Middle Aged
Pancreatitis, Chronic metabolism
Protons
Urinalysis methods
Validation Studies as Topic
Young Adult
Magnetic Resonance Spectroscopy methods
Metabolomics methods
Pancreatitis, Chronic diagnosis
Pancreatitis, Chronic urine
Subjects
Details
- Language :
- English
- ISSN :
- 1590-8577
- Volume :
- 14
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- JOP : Journal of the pancreas
- Publication Type :
- Academic Journal
- Accession number :
- 23474563
- Full Text :
- https://doi.org/10.6092/1590-8577/1294