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Gene expression of apoptosis-related genes, stress protein and antioxidant enzymes in hemocytes of white shrimp Litopenaeus vannamei under nitrite stress.
- Source :
-
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP [Comp Biochem Physiol C Toxicol Pharmacol] 2013 May; Vol. 157 (4), pp. 366-71. Date of Electronic Publication: 2013 Mar 06. - Publication Year :
- 2013
-
Abstract
- Apoptotic cell ratio and mRNA expression of caspase-3, cathepsin B (CTSB), heat shock protein 70 (HSP70), manganese superoxide dismutase (MnSOD), catalase (CAT), glutathione peroxidase (GPx) and thioredoxin (TRx) in hemocytes of white shrimp Litopenaeus vannamei exposed to nitrite-N (20 mg/L) was investigated at different stress time (0, 4, 8, 12, 24, 48 and 72 h). The apoptotic cell ratio and mRNA expression level of CTSB were significantly increased in shrimp exposed to nitrite-N for 48 and 72 h. Caspase-3 mRNA expression level significantly increased by 766.50% and 1811.16% for 24 and 48 h exposure, respectively. HSP70 expression level significantly increased at 8 and 72 h exposure. MnSOD mRNA expression in hemocytes up-regulated at 8 and 48 h, while CAT mRNA expression level increased at 24 and 48 h. GPx expression showed a trend that increased first and then decreased. Significant increases of GPx expression were observed at 8 and 12 h exposure. Expression level of TRx reached its highest level after 48 h exposure. These results suggest that nitrite exposure induces expression of apoptosis-related genes in hemocytes, and subsequently caused hemocyte apoptosis. Meanwhile, expression levels of HSP70 and antioxidant enzymes up-regulated to protect the hemocyte against nitrite stress.<br /> (Copyright © 2013 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Caspase 3 metabolism
Cathepsin B genetics
Cathepsin B metabolism
Environmental Exposure analysis
Glutathione Peroxidase metabolism
HSP70 Heat-Shock Proteins genetics
HSP70 Heat-Shock Proteins metabolism
Hemocytes drug effects
Oxidative Stress
RNA, Messenger genetics
RNA, Messenger metabolism
Superoxide Dismutase metabolism
Time Factors
Antioxidants metabolism
Apoptosis
Gene Expression Regulation, Enzymologic
Hemocytes enzymology
Nitrites pharmacology
Penaeidae drug effects
Penaeidae enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 1532-0456
- Volume :
- 157
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
- Publication Type :
- Academic Journal
- Accession number :
- 23474501
- Full Text :
- https://doi.org/10.1016/j.cbpc.2013.03.001