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Evidence for cross-talk between the LH receptor and LH during implantation in mice.
- Source :
-
Reproduction, fertility, and development [Reprod Fertil Dev] 2013; Vol. 25 (3), pp. 511-22. - Publication Year :
- 2013
-
Abstract
- The present study investigated the first interaction that occurs between the blastocyst and endometrium during implantation. Given the ethical objections to studying implantation in humans, a mouse model was used to study the dialogue between luteinising hormone (LH) and luteinising hormone receptor (LHCGR). Several studies performed on LHCGR-knockout mice have generated controversy regarding the importance of the dialogue between LH and LHCGR during implantation. There has been no demonstration of a bioactive LH-like signal produced by the murine blastocyst. The first aim of the present study was to examine and quantify, using radioimmunoassay, the generation of a bioactive LH signal by the murine blastocyst. We went on to examine and quantify endometrial Lhcgr expression to validate the mouse model. Expression of LHCGR in mouse uteri was demonstrated using immunohistochemistry and western blot analysis. To quantify the expression of Lh in the mouse blastocyst and Lhcgr in the endometrium, reverse transcription-polymerase chain reaction (RT-PCR) and real-time quantitative (q) RT-PCR were performed. The results demonstrate that Lhcgr expression in BALB/c mouse endometrial epithelium is increased at the time of implantation and indicate that LHCGR may contribute to the implantation process. In support of this hypothesis, we identified a bioactive LH signal at the time of murine blastocyst implantation.
- Subjects :
- Animals
Cells, Cultured
Embryo Transfer
Endometrium cytology
Estrous Cycle blood
Estrous Cycle metabolism
Estrus blood
Estrus metabolism
Female
Gene Expression Regulation, Developmental
Glycoprotein Hormones, alpha Subunit blood
Granulosa Cells cytology
Granulosa Cells metabolism
Leydig Cells cytology
Leydig Cells metabolism
Luteinizing Hormone, beta Subunit blood
Luteinizing Hormone, beta Subunit genetics
Male
Mice
Mice, Inbred Strains
Pregnancy
Receptors, LH genetics
Blastocyst metabolism
Embryo Implantation
Endometrium metabolism
Glycoprotein Hormones, alpha Subunit metabolism
Luteinizing Hormone, beta Subunit metabolism
Receptors, LH metabolism
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 1031-3613
- Volume :
- 25
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Reproduction, fertility, and development
- Publication Type :
- Academic Journal
- Accession number :
- 23464498
- Full Text :
- https://doi.org/10.1071/RD11241