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Correlation between Sorangium cellulosum subgroups and their potential for secondary metabolite production.
- Source :
-
Journal of microbiology and biotechnology [J Microbiol Biotechnol] 2013 Mar; Vol. 23 (3), pp. 297-303. - Publication Year :
- 2013
-
Abstract
- Phylogenetic analysis of the groEL1 and xynB1 gene sequences from Sorangium cellulosum strains isolated in Korea previously revealed the existence of at least 5 subgroups (A-E). In the present study, we used sequence analysis of polymerase chain reaction-amplified biosynthetic genes of strains from the 5 subgroups to indicate correlations between S. cellulosum subgroups and their secondary metabolic gene categories. We detected putative biosynthetic genes for disorazol, epothilone, ambruticin, and soraphen in group A, group C, group D, and group E strains, respectively. With the exception of KYC3204, culture extracts from group A, group B, and group C strains exhibited no noticeable antimicrobial inhibitory activities. By contrast, culture extracts from group D strains inhibited the growth of Candida albicans, whereas culture extracts from group E strains inhibited the growth of C. albicans and Staphylococcus aureus. High performance liquid chromatography analysis of the culture extracts from the strains of each subgroup revealed unique peak patterns. Our findings indicate the existence of at least 5 subgroups of S. cellulosum strains, each of which has the potential to produce a unique set of secondary metabolites.
- Subjects :
- Anti-Infective Agents analysis
Anti-Infective Agents pharmacology
Biological Products pharmacology
Biosynthetic Pathways genetics
Candida albicans drug effects
Candida albicans growth & development
Chaperonin 60 genetics
Chromatography, High Pressure Liquid
Cluster Analysis
DNA, Bacterial chemistry
DNA, Bacterial genetics
Endo-1,4-beta Xylanases genetics
Korea
Molecular Sequence Data
Myxococcales genetics
Myxococcales isolation & purification
Phylogeny
Polymerase Chain Reaction
Sequence Analysis, DNA
Staphylococcus aureus drug effects
Staphylococcus aureus growth & development
beta-Glucosidase genetics
Biological Products analysis
Myxococcales classification
Myxococcales metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1738-8872
- Volume :
- 23
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of microbiology and biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 23462001
- Full Text :
- https://doi.org/10.4014/jmb.1210.10054